Construct: ORF TRCN0000470480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008725.1_s317c1
- Derived from:
- ccsbBroadEn_12989
- DNA Barcode:
- TGCTCCCTCGAACATTCTGATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAPRT (93100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_145201.6 | 95.4% | 95.5% | 1_72del;913C>T |
| 2 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_001286829.2 | 93% | 93.1% | 1_72del;913C>T;1404_1405ins39 |
| 3 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_001363145.1 | 90.4% | 90.5% | 1_72del;913C>T;1105_1106ins81 |
| 4 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013976.2 | 84% | 84.1% | 1_72del;684_902del;1132C>T |
| 5 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013977.2 | 78.8% | 74.3% | (many diffs) |
| 6 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013975.2 | 74.4% | 70.4% | (many diffs) |
| 7 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013978.2 | 73.2% | 69.8% | (many diffs) |
| 8 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | NM_001363146.1 | 60.2% | 60.3% | 0_1ins612;229C>T |
| 9 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_017013979.2 | 52.2% | 48.1% | (many diffs) |
| 10 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_024447332.1 | 47.6% | 43.8% | (many diffs) |
| 11 | human | 93100 | NAPRT | nicotinate phosphoribosyltr... | XM_024447333.1 | 47.6% | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gttgggctat tggcgcgcgg gccgggcgcg ggacgccgcc gagttcgagc 121 tcttcttccg ccgctgcccg ttcggcggcg ccttcgcctt ggccgccggc ttgcgcgact 181 gtgtgcgctt cctgcgcgcc ttccgcctgc gggacgccga cgtgcagttc ctggcctcgg 241 tgctgccccc agacacggat cctgcgttct tcgagcacct tcgggccctc gactgctccg 301 aggtgacggt gcgagccctg cccgagggct ccctcgcctt ccccggagtg ccgctcctgc 361 aggtgtccgg gccgctcctg gtggtgcagc tgctggagac accgctgctc tgcctggtca 421 gctacgccag cctggtggcc accaacgcag cgcggcttcg cttgatcgca gggccagaga 481 agcggctgct agagatgggc ctgaggcggg ctcagggccc cgatgggggc ctgacagcct 541 ccacctacag ctacctgggc ggcttcgaca gcagcagcaa cgtgctagcg ggccagctgc 601 gaggtgtgcc ggtggccggg accctggccc actccttcgt cacttccttt tcaggcagcg 661 aggtgccccc tgacccgatg ttggcgccag cagctggtga gggccctggg gtggacctgg 721 cggccaaagc ccaggtgtgg ctggagcagg tgtgtgccca cctggggctg ggggtgcagg 781 agccgcatcc aggcgagcgg gcagcctttg tggcctatgc cttggctttt ccccgggcct 841 tccagggcct cctggacacc tacagcgtgt ggaggagtgg tctccccaac ttcctagcag 901 tcgccttggc cctgggagag ctgggctacc gggcagtggg cgtgaggctg gacagtggtg 961 acctgctaca gcaggctcag gagatccgca aggtcttccg agctgctgca gcccagttcc 1021 aggtgccctg gctggagtca gtcctcatcg tagtcagcaa caacattgac gaggaggcgc 1081 tggcccgact ggcccaggag ggcagtgagg tgaatgtcat tggcattggc accagtgtgg 1141 tcacctgccc ccaacagcct tcccTGGGTG GCGTCTATAA GCTGGTGGCC GTGGGGGGCC 1201 AGCCACGAAT GAAGCTGACC GAGGACCCCG AGAAGCAGAC GTTGCCTGGG AGCAAGGCTG 1261 CTTTCCGGCT CCTGGGCTCT GACGGGTCTC CACTCATGGA CATGCTGCAG TTAGCAGAAG 1321 AGCCAGTGCC ACAGGCTGGG CAGGAGCTGA GGGTGTGGCC TCCAGGGGCC CAGGAGCCCT 1381 GCACCGTGAG GCCAGCCCAG GTGGAGCCAC TACTGCGGCT CTGCCTCCAG CAGGGACAGC 1441 TGTGTGAGCC GCTCCCATCC CTGGCAGAGT CTAGAGCCTT GGCCCAGCTG TCCCTGAGCC 1501 GACTCAGCCC TGAGCACAGG CGGCTGCGGA GCCCTGCACA GTACCAGGTG GTGCTGTCCG 1561 AGAGGCTGCA GGCCCTGGTG AACAGTCTGT GTGCGGGGCA GTCCCCCTGC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGATGCTCCC TCGAACATTC TGATGCAACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt