Construct: ORF TRCN0000470481

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003790.1_s317c1
Derived from:
ccsbBroadEn_14133
DNA Barcode:
GACTAAGTTATTCCCTTTCAAGCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SSUH2 (51066)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470481
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51066 SSUH2 ssu-2 homolog NM_001256749.2 99.8% 99.6% 5C>N
2 human 51066 SSUH2 ssu-2 homolog NM_015931.3 85.4% 85.2% 1_153del;158C>N
3 human 51066 SSUH2 ssu-2 homolog XM_017006538.1 85.4% 85.2% 1_153del;158C>N
4 human 51066 SSUH2 ssu-2 homolog XM_017006539.1 85.4% 85.2% 1_153del;158C>N
5 human 51066 SSUH2 ssu-2 homolog XM_017006540.1 85.4% 85.2% 1_153del;158C>N
6 human 51066 SSUH2 ssu-2 homolog XM_017006541.1 85.4% 85.2% 1_153del;158C>N
7 human 51066 SSUH2 ssu-2 homolog XM_017006542.1 85.4% 85.2% 1_153del;158C>N
8 human 51066 SSUH2 ssu-2 homolog NM_001256748.2 80.4% 80.2% 1_219del;224C>N
9 human 51066 SSUH2 ssu-2 homolog XM_017006534.1 64.8% 64.7% 1_489del;494C>N
10 human 51066 SSUH2 ssu-2 homolog XM_017006533.1 62.4% 62.3% 1_543del;548C>N
11 human 51066 SSUH2 ssu-2 homolog XM_017006527.1 61.5% 61.4% 1_564del;569C>N
12 human 51066 SSUH2 ssu-2 homolog XM_017006528.1 61.5% 61.4% 1_564del;569C>N
13 human 51066 SSUH2 ssu-2 homolog XM_017006529.1 61.5% 61.4% 1_564del;569C>N
14 human 51066 SSUH2 ssu-2 homolog XM_017006530.1 61.5% 61.4% 1_564del;569C>N
15 human 51066 SSUH2 ssu-2 homolog XM_017006531.1 61.5% 61.4% 1_564del;569C>N
16 human 51066 SSUH2 ssu-2 homolog XM_017006525.1 58.4% 58.3% 1_642del;647C>N
17 human 51066 SSUH2 ssu-2 homolog XM_017006526.1 58.4% 58.3% 1_642del;647C>N
18 human 51066 SSUH2 ssu-2 homolog XM_017006524.1 58.3% 58.2% 1_645del;650C>N
19 human 51066 SSUH2 ssu-2 homolog XM_017006521.1 57.3% 57.2% 1_672del;677C>N
20 human 51066 SSUH2 ssu-2 homolog XM_017006522.1 57.3% 57.2% 1_672del;677C>N
21 human 51066 SSUH2 ssu-2 homolog XM_017006523.1 57.3% 57.2% 1_672del;677C>N
22 human 51066 SSUH2 ssu-2 homolog XM_017006520.1 55.4% 55.3% 1_726del;731C>N
23 human 51066 SSUH2 ssu-2 homolog XM_017006519.1 55% 54.9% 1_738del;743C>N
24 human 51066 SSUH2 ssu-2 homolog XM_017006518.1 54.7% 54.6% 1_747del;752C>N
25 human 51066 SSUH2 ssu-2 homolog XM_011533774.1 54% 53.9% 1_768del;773C>N
26 human 51066 SSUH2 ssu-2 homolog XM_017006511.1 54% 53.9% 1_768del;773C>N
27 human 51066 SSUH2 ssu-2 homolog XM_017006512.1 54% 53.9% 1_768del;773C>N
28 human 51066 SSUH2 ssu-2 homolog XM_017006513.1 54% 53.9% 1_768del;773C>N
29 human 51066 SSUH2 ssu-2 homolog XM_017006514.1 54% 53.9% 1_768del;773C>N
30 human 51066 SSUH2 ssu-2 homolog XM_017006515.1 54% 53.9% 1_768del;773C>N
31 human 51066 SSUH2 ssu-2 homolog XM_017006516.1 54% 53.9% 1_768del;773C>N
32 human 51066 SSUH2 ssu-2 homolog XM_017006517.1 54% 53.9% 1_768del;773C>N
33 human 51066 SSUH2 ssu-2 homolog XM_017006510.1 53.2% 53.1% 1_792del;797C>N
34 human 51066 SSUH2 ssu-2 homolog NR_046358.2 48.1% 1_507del;512C>N;1414_1881del
35 human 51066 SSUH2 ssu-2 homolog XM_017006532.1 39.2% 38.3% (many diffs)
36 human 51066 SSUH2 ssu-2 homolog XM_017006535.1 32.2% 32.1% 1_792del;797C>N;1341_1342ins357
37 human 51066 SSUH2 ssu-2 homolog XM_017006536.2 29.6% 27.2% (many diffs)
38 human 51066 SSUH2 ssu-2 homolog XM_017006537.1 22.2% 21.7% (many diffs)
39 human 51066 SSUH2 ssu-2 homolog XR_001740162.1 14.5% (many diffs)
40 human 51066 SSUH2 ssu-2 homolog XR_001740164.1 8.8% (many diffs)
41 human 51066 SSUH2 ssu-2 homolog XR_001740163.1 7.7% (many diffs)
42 human 51066 SSUH2 ssu-2 homolog XR_001740165.1 6.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
972
ORF length:
906
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgan ggaggaggtg gcccgggaag ccctcctcag ctttgtggac tctaagtgct
121gctacagcag cacggtggct ggagacctcg tcatccagga gctgaagcgg cagaccctct
181gcaggtaccg tctggagacc tttagtgaat ccaggataag cgagtggaca tttcaaccct
241ttactaacca ctctgtggat gggccgcaaa gaggcgcctc ccccaggctc tgggacatca
301aggttcaagg tcctccgatg tttcaggaag acaccaggaa gttccaggtc cctcactcgt
361cactggtcaa ggaatgccac aaatgccatg ggcgtgggcg gtacaagtgc agcggctgcc
421acggggcggg cacggtgcgg tgcccatcct gctgcggagc caagcgcaaa gccaagcagt
481cccggagatg tcagctgtgc gcggggtccg gcaggcgaag atgcagcact tgctcaggga
541gagggaacaa gacctgcgcc acctgcaagg gggagaagaa gctgttgcac ttcatccagc
601ttGTCATCAT GTGGAAGAAC AGCTTGTTTG AGTTTGTGTC TGAGCACCGG CTCAACTGCC
661CCAGGGAGCT CCTTGCTAAA GCCAAAGGAG AAAACCTCTT TAAGGATGAA AACTCGGTGG
721TGTACCCCAT CGTGGACTTC CCTCTGCGAG ACATCTCTCT TGCCTCCCAG AGGGGCATTG
781CAGAGCACAG CGCTGCCTTG GCCTCCCGTG CCCGCGTCCT GCAGCAGCGC CAGACCATTG
841AGCTGATCCC CCTCACAGAA GTTCACTATT GGTACCAAGG AAAGACTTAT GTCTACTACA
901TCTATGGCAC TGACCACCAG GTGTATGCGG TGGACTATCC TGAGCGGTAT TGCTGTGGCT
961GTACCATCGT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1021ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1081CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAC TAAGTTATTC CCTTTCAAGC
1141GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)