Construct: ORF TRCN0000470501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008653.1_s317c1
- Derived from:
- ccsbBroadEn_10980
- DNA Barcode:
- TTTGTCGTAGTGGCAGAGCGTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYCL (4610)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470501
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4610 | MYCL | MYCL proto-oncogene, bHLH t... | NM_005376.5 | 87.2% | 87.2% | 1_90del |
| 2 | human | 4610 | MYCL | MYCL proto-oncogene, bHLH t... | NM_001033081.3 | 49.7% | 44.7% | (many diffs) |
| 3 | human | 4610 | MYCL | MYCL proto-oncogene, bHLH t... | NM_001033082.3 | 46% | 41.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 684
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctacgactcg taccagcact atttctacga ctatgactgc ggggaggatt 121 tctaccgctc cacggcgccc agcgaggaca tctggaagaa attcgagctg gtgccatcgc 181 cccccacgtc gccgccctgg ggcttgggtc ccggcgcagg ggacccggcc cccgggattg 241 gtcccccGGA GCCGTGGCCC GGAGGGTGCA CCGGAGACGA AGCGGAATCC CGGGGCCACT 301 CGAAAGGCTG GGGCAGGAAC TACGCCTCCA TCATACGCCG TGACTGCATG TGGAGCGGCT 361 TCTCGGCCCG GGAACGGCTG GAGAGAGCTG TGAGCGACCG GCTCGCTCCT GGCGCGCCCC 421 GGGGGAACCC GCCCAAGGCG TCCGCCGCCC CGGACTGCAC TCCCAGCCTC GAAGCCGGCA 481 ACCCGGCGCC CGCCGCCCCC TGTCCGCTGG GCGAACCCAA GACCCAGGCC TGCTCCGGGT 541 CCGAGAGCCC AAGCGACTCG GGTAAGGACC TCCCCGAGCC ATCCAAGAGG GGGCCACCCC 601 ATGGGTGGCC AAAGCTCTGC CCCTGCCTGA GGTCAGGCAT TGGCTCTTCT CAAGCTCTTG 661 GGCCATCTCC GCCTCTCTTT GGCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTGTCGTAGT 841 GGCAGAGCGT GAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att