Construct: ORF TRCN0000470520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002681.1_s317c1
- Derived from:
- ccsbBroadEn_05139
- DNA Barcode:
- GGTCCCGAACCTGGGCCTTGGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM46 (166863)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 166863 | RBM46 | RNA binding motif protein 46 | NM_144979.5 | 100% | 100% | |
| 2 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_011531692.2 | 100% | 100% | |
| 3 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_011531693.2 | 100% | 100% | |
| 4 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_011531694.2 | 100% | 100% | |
| 5 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_011531695.3 | 100% | 100% | |
| 6 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_011531696.2 | 100% | 100% | |
| 7 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_011531697.2 | 89.5% | 86.8% | (many diffs) |
| 8 | human | 166863 | RBM46 | RNA binding motif protein 46 | NM_001277173.1 | 88% | 87.6% | (many diffs) |
| 9 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_006714120.1 | 88% | 87.6% | (many diffs) |
| 10 | human | 166863 | RBM46 | RNA binding motif protein 46 | NM_001277171.2 | 87.6% | 87.9% | (many diffs) |
| 11 | human | 166863 | RBM46 | RNA binding motif protein 46 | XM_006714119.2 | 87.6% | 87.9% | (many diffs) |
| 12 | mouse | 633285 | Rbm46 | RNA binding motif protein 46 | NM_001146328.2 | 90.6% | 96.4% | (many diffs) |
| 13 | mouse | 633285 | Rbm46 | RNA binding motif protein 46 | XM_006501850.3 | 90.6% | 96.4% | (many diffs) |
| 14 | mouse | 633285 | Rbm46 | RNA binding motif protein 46 | XM_006501851.1 | 90.6% | 96.4% | (many diffs) |
| 15 | mouse | 633285 | Rbm46 | RNA binding motif protein 46 | XM_011240188.1 | 90.6% | 96.4% | (many diffs) |
| 16 | mouse | 633285 | Rbm46 | RNA binding motif protein 46 | NM_001277170.1 | 81% | 85.7% | (many diffs) |
| 17 | mouse | 633285 | Rbm46 | RNA binding motif protein 46 | XM_017319680.1 | 81% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1665
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgaagaaaat atagatggaa caaatggatg cagtaaagtt cgaactggta 121 ttcagaatga agcagcatta cttgctttga tggaaaagac tggttacaac atggttcagg 181 aaaatggaca aaggaaattt ggcggtcctc ctccaggttg ggaaggtcca cctccaccta 241 gaggctgtga agtttttgta ggaaaaatac ctcgtgatat gtatgaagat gagttagttc 301 ctgtatttga aagagctggg aagatatatg aatttcgact tatgatggaa tttagtggtg 361 aaaatcgagg ttatgctttt gtgatgtaca ctacaaaaga agaagcccaa ttagccatca 421 gaattcttaa taattatgaa attcgaccag ggaagtttat tggtgtgtgt gtaagcctgg 481 ataattgtag attatttatt ggagctattc ccaaggaaaa gaagaaagaa gaaattttag 541 atgaaatgaa gaaagttaca gaaggagttg tagatgtcat tgtttatcca agtgcaactg 601 ataagaccaa aaatcgtggt tttgcatttg tggaatatga atctcacaga gctgctgcta 661 tggcaaggag gaaactaatt ccaggaacat tccaactatg gggccacacc attcaggtag 721 attgggctga cccagagaaa gaggtggatg aggaaaccat gcagagagtt aaagttcttt 781 atgtaagaaa tttaatgatc tcaactacag aggaaacaat taaagcagaa ttcaataaat 841 ttaagcctgg tgcagttgaa cgggtaaaga aacttagaga ttatgctttt gttcactttt 901 tcaaccgaga agatgcagtg gctgccatgt ctgttatgaa tggaaaatgc attgatggag 961 caagtattga ggtaacacta gctaaaccag taaataaaga aaacacttgg agacagcatc 1021 ttaatggtca gattagtcca aattctgaaa atctgattgt gtttgctaac aaagaagaga 1081 gccacccaaa aactctaggc aagctgccaa ctcttcctgc tcgtctcaat ggtcagcata 1141 gcccaagtcc gcctgaagtt gaaagatgca cttacccttt ttatcctgga acaaagctta 1201 ctccaattag tatgtattct ttaaaatcca atcattttaa ttctgcagta atgcatttgG 1261 ATTATTACTG CAACAAAAAT AACTGGGCAC CACCAGAATA TTATTTATAT TCAACAACAA 1321 GTCAAGATGG GAAAGTACTC TTGGTGTATA AGATAGTTAT TCCTGCTATT GCAAATGGAT 1381 CCCAGAGTTA CTTCATGCCA GACAAACTCT GTACTACGTT AGAAGATGCA AAGGAACTGG 1441 CAGCCCAGTT TACATTACTT CATTTGGACT ACAATTTCCA TCGCAGCTCA ATAAATAGTC 1501 TTTCCCCTGT TAGTGCTACC CTCTCTTCTG GGACTCCCAG CGTGCTTCCT TATACTTCAA 1561 GGCCTTATTC TTATCCAGGC TATCCTTTGT CACCAACAAT ATCACTTGCT AATGGCAGCC 1621 ATGTTGGACA GCGGCTATGT ATCTCCAATC AGGCCTCCTT CTTCTGCCCA ACTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA GGTCCCGAAC CTGGGCCTTG GTGTACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt