Construct: ORF TRCN0000470536
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008798.1_s317c1
- Derived from:
- ccsbBroadEn_15709
- DNA Barcode:
- CTCCCTTGAATAGCACGCCTCGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BTN2A2 (10385)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470536
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197238.2 | 99.9% | 98.8% | 996delA |
| 2 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714955.3 | 94.6% | 92.3% | (many diffs) |
| 3 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010168.2 | 94.6% | 92.3% | (many diffs) |
| 4 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010169.1 | 94.6% | 92.3% | (many diffs) |
| 5 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197234.3 | 78.7% | 73% | (many diffs) |
| 6 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_078476.4 | 78.1% | 72.9% | (many diffs) |
| 7 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197240.2 | 68% | 66.8% | (many diffs) |
| 8 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197237.2 | 63% | 62.9% | (many diffs) |
| 9 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_006995.5 | 63% | 62.9% | (many diffs) |
| 10 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248797.3 | 63% | 62.9% | (many diffs) |
| 11 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714953.4 | 63% | 62.9% | (many diffs) |
| 12 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514228.2 | 63% | 62.9% | (many diffs) |
| 13 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_007049.5 | 51.7% | 47.6% | (many diffs) |
| 14 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514229.2 | 51.2% | 51% | (many diffs) |
| 15 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010166.2 | 51.2% | 51% | (many diffs) |
| 16 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_024446302.1 | 51.2% | 51% | (many diffs) |
| 17 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197233.2 | 41.3% | 37.5% | (many diffs) |
| 18 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_181531.3 | 40.9% | 40.7% | (many diffs) |
| 19 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248798.4 | 40.9% | 40.7% | (many diffs) |
| 20 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010167.1 | 40.9% | 40.7% | (many diffs) |
| 21 | human | 54718 | BTN2A3P | butyrophilin subfamily 2 me... | NR_027795.1 | 33.1% | (many diffs) | |
| 22 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197239.2 | 23% | 22.7% | (many diffs) |
| 23 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514231.3 | 23% | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga accagctgct gctctgcact tctccctgcc agcctccctc ctcctcctcc 121 tgctcctcct ccttctcagc ctgtgtgcac tggtctcagc ccagtttact gtcgtggggc 181 cagctaatcc catcctggcc atggtgggag aaaacactac attacgctgc catctgtcac 241 ccgagaaaaa tgctgaggac atggaggtgc ggtggttccg gtctcagttc tcccccgcag 301 tgtttgtgta taagggtggg agagagagaa cagaggagca gatggaggag taccggggaa 361 gaatcacctt tgtgagcaaa gacatcaaca ggggcagcgt ggccctggtc atacataacg 421 tcacagccca ggagaatggg atctaccgct gttacttcca agaaggcagg tcctacgatg 481 aggccatcct acgcctcgtg gtggcaggcc ttgggtctaa gcccctcatt gaaatcaagg 541 cccaagagga tgggagcatc tggctggagt gcatatctgg agggtggtac ccagagcccc 601 tcacagtgtg gagggacccc tacggtgagg ttgtgcccgc ccTGAAGGAG GTTTCCATCG 661 CTGATGCTGA CGGCCTCTTC ATGGTCACCA CAGCTGTGAT CATCAGAGAC AAGTATGTGA 721 GGAATGTGTC CTGCTCTGTC AACAACACCC TGCTCGGCCA GGAGAAGGAA ACTGTCATTT 781 TTATTCCAGA ATCCTTTATG CCCAGCGCAT CTCCCTGGAT GGTGGCCCTA GCTGTCATCC 841 TGACCGCATC TCCCTGGATG GTGTCCATGA CTGTCATCCT GGCTGTTTTC ATCATCTTCA 901 TGGCTGTCAG CATCTGTTGC ATCAAGAAAC TTCAAAGGGA AAAAAAGATT CTGTCAGGGG 961 AAAAGAAAGT TGAACAAGAG GAAAAAGAAA TTGCACAGCA ACTTCAAGAA GAATTGCGAT 1021 GGAGAAGAAC ATTCTTACAT GCTGATGTCA ACCTGACTGG TTAAGGAATA CCTATCCAAC 1081 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1141 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1201 ATCTTGTGGA AAGGACGACT CCCTTGAATA GCACGCCTCG CGACGCGTTA AGTCgacaat 1261 caacctctgg attacaaaat ttgtgaaaga tt