Construct: ORF TRCN0000470541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016410.1_s317c1
- Derived from:
- ccsbBroadEn_15176
- DNA Barcode:
- TATAGAGTAGCGGTCTTGGTCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUAK2 (81788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81788 | NUAK2 | NUAK family kinase 2 | NM_030952.3 | 99.9% | 100% | 585T>C |
| 2 | human | 81788 | NUAK2 | NUAK family kinase 2 | XM_005245515.4 | 60.6% | 60.6% | 0_1ins741 |
| 3 | mouse | 74137 | Nuak2 | NUAK family, SNF1-like kina... | NM_028778.4 | 83.7% | 85.6% | (many diffs) |
| 4 | mouse | 74137 | Nuak2 | NUAK family, SNF1-like kina... | NM_001195025.1 | 82.7% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1950
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtcgctggtt ttcgcgcggc gctccggccc cactccctcg gccgcagagc 121 tagcccggcc gctggcggaa gggctgatca agtcgcccaa gcccctaatg aagaagcagg 181 cggtgaagcg gcaccaccac aagcacaacc tgcggcaccg ctacgagttc ctggagaccc 241 tgggcaaagg cacctacggg aaggtgaaga aggcgcggga gagctcgggg cgcctggtgg 301 ccatcaagtc aatccggaag gacaaaatca aagatgagca agatctgatg cacatacgga 361 gggagattga gatcatgtca tcactcaacc accctcacat cattgccatc catgaagtgt 421 ttgagaacag cagcaagatc gtgatcgtca tggagtatgc cagccggggc gacctttatg 481 actacatcag cgagcggcag cagctcagtg agcgcgaagc taggcatttc ttccggcaga 541 tcgtctctgc cgtgcactat tgccatcaga acagagttgt ccaccgagat ctcaagctgg 601 agaacatcct cttggatgcc aatgggaata tcaagattgc tgacttcggc ctctccaacc 661 tctaccatca aggcaagttc ctgcagacat tctgtgggag ccccctctat gcctcgccag 721 agattgtcaa tgggaagccc tacacaggcc cagaggtgga cagctggtcc ctgggtgttc 781 tcctctacat cctggtgcat ggcaccatgc cctttgatgg gcatgaccat aagatcctag 841 tgaaacagat cagcaacggg gcctaccggg agccacctaa accctctgat gcctgtggcc 901 tgatccggtg gctgttgatg gtgaacccca cccgccgggc caccctggag gatgtggcca 961 gtcactggtg ggtcaactgg ggctacgcca cccgagtggg agagcaggag gctccgcatg 1021 agggtgggca ccctggcagt gactctgccc gcgcctccat ggctgactgg ctccggcgtt 1081 cctcccgccc cctcctggag aatggggcca aggtgtgcag cttcttcaag cagcatgcac 1141 ctggtggggg aagcaccacc cctggcctgg agcgccagca ttcgctcaag aagtcccgca 1201 aggagaatga catggcccag tctctccaca gtgacacggc tgatgacact gcccatcgcc 1261 ctggcaagag caacctcaag ctgccaaagg gcattctcaa gaagaaggtg tcagcctctg 1321 cagaaggggt acaggaggac cctccggagc tcagcccaat ccctgcgagc ccagggcagg 1381 ctgcccccct gctccccaag aagggcattc tcaagaagcc ccgacagcgc gagtctggct 1441 actactcctc tcccgagccc agtgaatctg gggagctctt ggacgcaggc gacgtgtttg 1501 tgagtgggga tcccaaggag cagaagcctc cgcaagcttc agggctgctc ctccatcgca 1561 aaggcatcct caaactcaat ggcaagttct cccagacagc cttggagctc gcggccccca 1621 ccaccTTCGG CTCCCTGGAT GAACTCGCCC CACCTCGCCC CCTGGCCCGG GCCAGCCGAC 1681 CCTCAGGGGC TGTGAGCGAG GACAGCATCC TGTCCTCTGA GTCCTTTGAC CAGCTGGACT 1741 TGCCTGAACG GCTCCCAGAG CCCCCACTGC GGGGCTGTGT GTCTGTGGAC AACCTCACGG 1801 GGCTTGAGGA GCCCCCCTCA GAGGGCCCTG GAAGCTGCCT GAGGCGCTGG CGGCAGGATC 1861 CTTTGGGGGA CAGCTGCTTT TCCCTGACAG ACTGCCAGGA GGTGACAGCG ACCTACCGAC 1921 AGGCACTGAG GGTCTGCTCA AAGCTCACCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1981 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2041 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATATAG 2101 AGTAGCGGTC TTGGTCACCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2161 tgaaagatt