Construct: ORF TRCN0000470544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011312.3_s317c1
- Derived from:
- ccsbBroadEn_15293
- DNA Barcode:
- TGCAACACAGGTACACGGGACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IPMK (253430)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253430 | IPMK | inositol polyphosphate mult... | NM_152230.5 | 100% | 100% | |
| 2 | human | 253430 | IPMK | inositol polyphosphate mult... | XM_011539565.3 | 99.2% | 99.2% | 276_284delGGTAAGTGA |
| 3 | human | 253430 | IPMK | inositol polyphosphate mult... | XM_017016013.2 | 78.4% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1314
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aacagagcca ccatcccccc tccgggtcga ggcgccgggc cccccagaaa 121 tgcggacctc accggcgatc gagtccaccc ctgagggcac cccgcagccg gcgggcggca 181 gactccgctt cctcaacggc tgcgtgcccc tctcgcatca ggtggccggg cacatgtacg 241 ggaaggacaa agtgggtata ctgcaacatc cagatggcac agttttgaaa cagttacaac 301 cacctccaag gggcccaaga gagctggaat tctataatat ggtttatgct gctgactgtt 361 ttgatggtgt tcttctagag ctacgaaaat atttgccaaa atattatggc atctggtcac 421 ctcccactgc accaaacgat ttatacctaa aactggaaga tgtgacccat aaatttaata 481 agccctgtat aatggatgta aagatagggc aaaaaagcta tgatcctttt gcctcatctg 541 agaagattca gcaacaggtc agcaagtacc cattaatgga agagattggg ttcttggtgc 601 ttggcatgag ggtttatcat gttcattccg atagctatga gacagaaaac cagcattacg 661 gaagaagctt aacaaaagaa actataaagg atggagtctc cagatttttt cataatgggt 721 actgcttaag aaaagatgct gttgctgcca gtattcagaa gattgagaaa attctgcagt 781 ggtttgaaaa ccagaagcag cttaattttt acgcaagttc attactcttt gtttatgaag 841 gttcatctca gccaaccact acaaaattga atgacagaac tttggCAGAA AAGTTTTTGT 901 CCAAAGGACA ACTGTCAGAC ACAGAAGTAC TAGAGTACAA TAATAACTTT CATGTGTTAA 961 GTTCCACAGC TAATGGAAAA ATAGAGTCTT CAGTGGGCAA AAGCTTGTCC AAGATGTATG 1021 CGCGTCACAG GAAAATATAT ACAAAAAAGC ATCACAGTCA GACTTCATTG AAAGTTGAAA 1081 ATCTGGAGCA AGACAATGGG TGGAAAAGCA TGTCACAGGA ACATTTAAAT GGAAATGTAC 1141 TTTCCCAACT GGAAAAAGTT TTCTACCATC TTCCCACTGG TTGCCAAGAG ATTGCTGAAG 1201 TAGAAGTGCG AATGATAGAT TTTGCTCATG TGTTCCCTAG CAACACAATA GATGAGGGAT 1261 ATGTTTATGG GCTAAAGCAT TTAATTTCTG TACTTCGAAG TATTTTAGAC AATTGCCCAA 1321 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1381 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1441 TATCTTGTGG AAAGGACGAT GCAACACAGG TACACGGGAC ACCACGCGTT AAGTCgacaa 1501 tcaacctctg gattacaaaa tttgtgaaag att