Transcript: Human NM_152230.5

Homo sapiens inositol polyphosphate multikinase (IPMK), mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
IPMK (253430)
Length:
6093
CDS:
284..1534

Additional Resources:

NCBI RefSeq record:
NM_152230.5
NBCI Gene record:
IPMK (253430)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001487069 GTATAATGGATGTAAAGATA pXPR_003 GGG 438 35% 4 1.0713 IPMK IPMK 78074
2 BRDN0001146121 ATGTACGGGAAGGACAAAGT pXPR_003 GGG 185 15% 1 0.9564 IPMK IPMK 78075
3 BRDN0001144988 TTTATGACAAGATTACCGTT pXPR_003 TGG 372 30% 3 -0.1893 IPMK IPMK 78076
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152230.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219804 GGTCAGCAAGTACCCATTAAT pLKO.1 775 CDS 100% 15.000 21.000 N IPMK n/a
2 TRCN0000196885 GCACCTTTAATGCTATGTAAA pLKO.1 1598 3UTR 100% 13.200 18.480 N IPMK n/a
3 TRCN0000195137 CAGAAGTACTAGAGTACAATA pLKO.1 1140 CDS 100% 13.200 10.560 N IPMK n/a
4 TRCN0000052602 CAGACTTCATTGAAAGTTGAA pLKO.1 1277 CDS 100% 4.950 3.960 N IPMK n/a
5 TRCN0000196360 GATATGTTTATGGGCTAAAGC pLKO.1 1476 CDS 100% 4.950 3.960 N IPMK n/a
6 TRCN0000052598 GCCCTGTATAATGGATGTAAA pLKO.1 700 CDS 100% 13.200 9.240 N IPMK n/a
7 TRCN0000052600 GCAAGTTCATTACTCTTTGTT pLKO.1 1031 CDS 100% 5.625 3.938 N IPMK n/a
8 TRCN0000219805 GGGTGGGCCAATCATAATGAA pLKO.1 1559 3UTR 100% 5.625 3.938 N IPMK n/a
9 TRCN0000052601 GCACCAAACGATTTATACCTA pLKO.1 647 CDS 100% 3.000 2.100 N IPMK n/a
10 TRCN0000052599 GCAACACAATAGATGAGGGAT pLKO.1 1458 CDS 100% 2.640 1.848 N IPMK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152230.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15293 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15293 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470544 TGCAACACAGGTACACGGGACACC pLX_317 34.6% 100% 100% V5 n/a
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