Construct: ORF TRCN0000470545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014486.1_s317c1
- Derived from:
- ccsbBroadEn_09671
- DNA Barcode:
- AAACACCCTCAGCGACATCTTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMYD1 (150572)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150572 | SMYD1 | SET and MYND domain contain... | NM_198274.4 | 99.9% | 100% | 337C>A |
| 2 | human | 150572 | SMYD1 | SET and MYND domain contain... | NM_001330364.2 | 97.2% | 97.1% | 337C>A;659_660ins39 |
| 3 | mouse | 12180 | Smyd1 | SET and MYND domain contain... | NM_001160127.1 | 89.5% | 94.4% | (many diffs) |
| 4 | mouse | 12180 | Smyd1 | SET and MYND domain contain... | NM_009762.2 | 87% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1539
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaataggg agaatggaga acgtggaggt cttcaccgct gagggcaaag 121 gaaggggtct gaaggccacc aaggagttct gggctgcaga tatcatcttt gctgagcggg 181 cttattccgc agtggttttt gacagccttg ttaattttgt gtgccacacc tgcttcaaga 241 ggcaggagaa gctccatcgc tgtgggcagt gcaagtttgc ccattactgc gaccgcacct 301 gccagaagga tgcttggctg aaccacaaga atgaatgttc ggccatcaag agatatggga 361 aggtgcccaa tgagaacatc aggctggcgg cgcgcatcat gtggagggtg gagagagaag 421 gcaccgggct cacggagggc tgcctggtgt ccgtggacga cttgcagaac cacgtggagc 481 actttgggga ggaggagcag aaggacctgc gggtggacgt ggacacattc ttgcagtact 541 ggccgccgca gagccagcag ttcagcatgc agtacatctc gcacatcttc ggagtgatta 601 actgcaacgg ttttactctc agtgatcaga gaggcctgca ggccgtgggc gtaggcatct 661 tccccaacct gggcctggtg aaccatgact gttggcccaa ctgtactgtc atatttaaca 721 atggcaatca tgaggcagtg aaatccatgt ttcataccca gatgagaatt gagctccggg 781 ccctaggcaa gatctcagaa ggagaggagc tgactgtgtc ctatattgac ttcctcaacg 841 ttagtgaaga acgcaagagg cagctgaaga agcagtacta ctttgactgc acatgtgaac 901 actgccagaa aaaactgaag gatgacctct tcctgggggt gaaagacaac cccaagccct 961 ctcaggaagt ggtgaaggag atgatacaat tctccaagga tacattggaa aagatagaca 1021 aggctcgttc cgagggtttg tatcatgagg ttgtgaaatt atgccgggag tgcctggaga 1081 agcaggagcc agtgtttgct gacaccaaca tctacatgct gcggatgctg agcattgttt 1141 cggaggtcct ttcctacctc caggccttTG AGGAGGCCTC GTTCTATGCC AGGAGGATGG 1201 TGGACGGCTA TATGAAGCTC TACCACCCCA ACAATGCCCA ACTGGGCATG GCCGTGATGC 1261 GGGCAGGGCT GACCAACTGG CATGCTGGTA ACATTGAGGT GGGGCACGGG ATGATCTGCA 1321 AAGCCTATGC CATTCTCCTG GTGACACACG GACCCTCCCA CCCCATCACT AAGGACTTAG 1381 AGGCCATGCG GGTGCAGACG GAGATGGAGC TACGCATGTT CCGCCAGAAC GAATTCATGT 1441 ACTACAAGAT GCGCGAGGCT GCCCTGAACA ACCAGCCCAT GCAGGTCATG GCCGAGCCCA 1501 GCAATGAGCC ATCCCCAGCT CTGTTCCACA AGAAGCAATT GCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGAAAACAC CCTCAGCGAC ATCTTATCAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt