Construct: ORF TRCN0000470554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007152.1_s317c1
- Derived from:
- ccsbBroadEn_01661
- DNA Barcode:
- TTTCGACTACCGCGCAAACTAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TFCP2 (7024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7024 | TFCP2 | transcription factor CP2 | NM_005653.5 | 100% | 100% | |
| 2 | human | 7024 | TFCP2 | transcription factor CP2 | NM_001173452.2 | 99.8% | 99.8% | 1469_1470insAGC |
| 3 | human | 7024 | TFCP2 | transcription factor CP2 | NM_001173453.2 | 89.6% | 89.6% | 562_563ins153;1316_1317insAGC |
| 4 | mouse | 21422 | Tfcp2 | transcription factor CP2 | NM_033476.3 | 88.3% | 96.6% | (many diffs) |
| 5 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_006520738.2 | 88.1% | 96.4% | (many diffs) |
| 6 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_017316540.1 | 88.1% | 96.4% | (many diffs) |
| 7 | mouse | 21422 | Tfcp2 | transcription factor CP2 | NM_001289603.1 | 87.9% | 96.2% | (many diffs) |
| 8 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_017316538.1 | 78.1% | 84.3% | (many diffs) |
| 9 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_017316537.1 | 77.9% | 84.1% | (many diffs) |
| 10 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_017316539.1 | 77.9% | 84.1% | (many diffs) |
| 11 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_017316541.1 | 53.6% | 57.2% | (many diffs) |
| 12 | mouse | 21422 | Tfcp2 | transcription factor CP2 | XM_017316542.1 | 47% | 50.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1572
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgggctctg aagctgcctc tggccgacga agtgattgaa tccgggttgg 121 tgcaggactt tgatgctagc ctgtccggga tcggccagga actgggtgct ggtgcctata 181 gcatgagtga tgtccttgca ttgcccattt ttaagcaaga agagtcgagt ttgcctcctg 241 ataatgagaa taaaatcctg ccttttcaat atgtgctttg tgctgctacc tctccagcag 301 tgaaactcca tgatgaaacc ctaacgtatc tcaatcaagg acagtcttat gaaattcgaa 361 tgctagacaa taggaaactt ggagaacttc cagaaattaa tggcaaattg gtgaagagta 421 tattccgtgt ggtgttccat gacagaaggc ttcagtacac tgagcatcag cagctagagg 481 gctggaggtg gaaccgacct ggagacagaa ttcttgacat agatatcccg atgtctgtgg 541 gtataatcga tcctagggct aatccaactc aactaaatac agtggagttc ctgtgggacc 601 ctgcaaagag gacatctgtg tttattcagg tgcactgtat tagcacagag ttcactatga 661 ggaaacatgg tggagaaaag ggggtgccat tccgagtaca aatagatacc ttcaaggaga 721 atgaaaacgg ggaatatact gagcacttac actcggccag ctgccagatc aaagttttca 781 agcccaaagg tgcagacaga aagcaaaaaa cggataggga aaaaatggag aaacgaacac 841 ctcatgaaaa ggagaaatat cagccttcct atgagacaac catactcaca gagtgttctc 901 catggcccga gatcacgtat gtcaataact ccccatcacc tggcttcaac agttcccata 961 gcagtttttc tcttggggaa ggaaatggtt caccaaacca ccagccagag ccaccccctc 1021 cagtcacaga taacctctta ccaacaacca cacctcagga agctcagcag tggttgcatc 1081 gaaatcgttt ttctacattc acaaggcttt tcacaaactt cTCAGGGGCA GATTTATTGA 1141 AATTAACTAG AGATGATGTG ATCCAAATCT GTGGCCCTGC AGATGGAATC AGACTTTTTA 1201 ATGCATTAAA AGGCCGGATG GTGCGTCCAA GGTTAACCAT TTATGTTTGT CAGGAATCAC 1261 TGCAGTTGAG GGAGCAGCAA CAACAGCAGC AGCAACAGCA GCAGAAGCAT GAGGATGGAG 1321 ACTCAAATGG TACTTTCTTC GTTTACCATG CTATCTATCT AGAAGAACTA ACAGCTGTTG 1381 AATTGACAGA AAAAATTGCT CAGCTTTTCA GCATTTCCCC TTGCCAGATC AGCCAGATTT 1441 ACAAGCAGGG GCCAACAGGA ATTCATGTGC TCATCAGTGA TGAGATGATA CAGAACTTTC 1501 AGGAAGAAGC ATGTTTTATT CTGGACACAA TGAAAGCAGA AACCAATGAT AGCTATCATA 1561 TCATACTGAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1621 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1681 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT CGACTACCGC GCAAACTAGC 1741 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t