Construct: ORF TRCN0000470555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009917.1_s317c1
- Derived from:
- ccsbBroadEn_01618
- DNA Barcode:
- AAACTTCGACCTTTGTGTATGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STXBP1 (6812)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001032221.5 | 100% | 100% | |
| 2 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374306.1 | 99.4% | 99.3% | 325_326insCTTGTCCAG |
| 3 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374309.1 | 97.6% | 97.6% | 0_1ins42 |
| 4 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374310.1 | 97.6% | 97.6% | 0_1ins42 |
| 5 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374311.1 | 97.6% | 97.6% | 0_1ins42 |
| 6 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374312.1 | 97.6% | 97.6% | 0_1ins42 |
| 7 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374313.1 | 96.1% | 95.7% | (many diffs) |
| 8 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_003165.5 | 96% | 95.8% | (many diffs) |
| 9 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374314.1 | 96% | 94.9% | (many diffs) |
| 10 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374307.1 | 93.7% | 93.5% | (many diffs) |
| 11 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374308.1 | 93.7% | 93.5% | (many diffs) |
| 12 | human | 6812 | STXBP1 | syntaxin binding protein 1 | NM_001374315.1 | 90.1% | 89.9% | (many diffs) |
| 13 | mouse | 20910 | Stxbp1 | syntaxin binding protein 1 | NM_009295.2 | 90.8% | 100% | (many diffs) |
| 14 | mouse | 20910 | Stxbp1 | syntaxin binding protein 1 | NM_001113569.1 | 87.4% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1848
- ORF length:
- 1782
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccccattggc ctcaaagctg ttgtcggaga gaagattatg catgatgtga 121 taaagaaggt caagaagaag ggggaatgga aggtgctggt ggtggatcag ttaagcatga 181 ggatgctgtc ctcctgctgc aagatgacag acatcatgac cgagggcata acgattgtgg 241 aagatatcaa taagcgcaga gagccgctcc ccagcctgga ggctgtgtat ctcatcactc 301 catccgagaa gtccgtccac tctctcatca gtgactttaa ggacccgccg actgctaaat 361 accgggctgc acacgtcttc ttcactgact cttgtccaga tgccctgttt aatgaactgg 421 taaaatcccg agcagccaaa gtcatcaaaa ctctgacgga aatcaatatt gcatttctcc 481 cgtatgaatc ccaggtctat tccttggact ctgctgactc tttccaaagc ttctacagtc 541 cccacaaggc tcagatgaag aatcctatac tggagcgcct ggcagagcag atcgcgaccc 601 tttgtgccac cctgaaggag tacccggctg tgcggtatcg gggggaatac aaggacaatg 661 ccctgctggc tcagctaatc caggacaagc tcgatgccta taaagctgat gatccaacaa 721 tgggggaggg cccagacaag gcacgctccc agctcctgat cctggatcga ggctttgacc 781 ccagctcccc tgtgctccat gaattgactt ttcaggctat gagttatgat ctgctgccta 841 tcgaaaatga tgtatacaag tatgagacca gcggcatcgg ggaggcacgg gtgaaggagg 901 tgctcctgga cgaggacgac gacctgtgga tagcactgcg ccacaagcac atcgcagagg 961 tgtcccagga agtcacccgg tctctgaaag atttttcttc tagcaagaga atgaatactg 1021 gagagaagac caccatgcgg gacctgtccc agatgctgaa gaagatgcct cagtaccaga 1081 aagagctcag caagtactcc acccacctgc accttgctga ggactgtatg aagcattacc 1141 aaggcaccgt agacaaactc tgccgagtgg agcaggacct ggccatgggc acagatgctg 1201 agggagagaa gatcaaggac cctatgcgag ccatcgtccc cattctgctg gatgccaatg 1261 tcagcactta tgacaaaatc cgcatcatcc ttctctacat ctttttgaag aatggcatca 1321 cggaggaaaa cctgaacaaa ctgatccagc acgcccagat acccccggag gatagtgaga 1381 tcatcaccaa catggctcac ctcggcgtgc ccatcgtcac cgattccacg ctgcgtcgcc 1441 ggagcaagcc ggagcggaag gaacgcatca gcgagcagac ctaccagctc tcacggtgga 1501 ctccgattat caaggacatc atggaggaca ctattgagga caaacttgac accaaacact 1561 acccttatat ctctacccgt tcctctgcct ccttcagcac caccgccgtc agcgcccgct 1621 atgggcactg gcataagaac aaggccccag gcgagtaccg cagtggcccc cgcctcatca 1681 ttttcatcct tgggggtgtg agcctgaaTG AGATGCGCTG CGCCTACGAG GTGACCCAGG 1741 CCAACGGAAA GTGGGAGGTG CTGATAGGAT CCACACACAT CCTCACCCCA CAGAAACTGC 1801 TGGACACACT GAAGAAACTG AATAAAACAG ATGAAGAAAT AAGCAGTTAC CCAACTTTCT 1861 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1921 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1981 GTGGAAAGGA CGAAAACTTC GACCTTTGTG TATGGTAACG CGTTAAGTCg acaatcaacc 2041 tctggattac aaaatttgtg aaagatt