Construct: ORF TRCN0000470558

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017576.1_s317c1
Derived from:
ccsbBroadEn_15422
DNA Barcode:
GCAGAACGATTTCGCCTGCAAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GABPB1 (2553)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470558
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2553 GABPB1 GA binding protein transcri... NM_002041.4 100% 100%
2 human 2553 GABPB1 GA binding protein transcri... NM_016655.4 96.6% 96.6% 578_579ins36
3 human 2553 GABPB1 GA binding protein transcri... NM_181427.3 96.6% 96.6% 578_579ins36
4 human 2553 GABPB1 GA binding protein transcri... NM_001320910.2 89.2% 88.1% (many diffs)
5 human 2553 GABPB1 GA binding protein transcri... NM_005254.6 89.2% 88.1% (many diffs)
6 human 2553 GABPB1 GA binding protein transcri... XM_005254274.4 89.2% 88.1% (many diffs)
7 human 2553 GABPB1 GA binding protein transcri... NM_001320915.2 88.9% 87.8% (many diffs)
8 human 2553 GABPB1 GA binding protein transcri... XM_024449886.1 88.9% 87.8% (many diffs)
9 human 2553 GABPB1 GA binding protein transcri... XM_024449885.1 88.5% 87.4% (many diffs)
10 human 2553 GABPB1 GA binding protein transcri... XM_024449883.1 88.1% 87% (many diffs)
11 human 2553 GABPB1 GA binding protein transcri... XM_024449884.1 87.8% 86.7% (many diffs)
12 human 2553 GABPB1 GA binding protein transcri... NM_016654.5 86.2% 85% (many diffs)
13 human 2553 GABPB1 GA binding protein transcri... XM_017022053.2 86.2% 85% (many diffs)
14 human 2553 GABPB1 GA binding protein transcri... XM_011521426.3 85.9% 84.8% (many diffs)
15 human 2553 GABPB1 GA binding protein transcri... XM_017022054.2 85.9% 84.8% (many diffs)
16 human 2553 GABPB1 GA binding protein transcri... XM_024449887.1 85.1% 84% (many diffs)
17 human 2553 GABPB1 GA binding protein transcri... XM_024449888.1 84.8% 83.7% (many diffs)
18 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_010249.2 89.4% 94.1% (many diffs)
19 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271470.1 89.1% 93.8% (many diffs)
20 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498762.1 85.3% 90.5% (many diffs)
21 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271468.1 79.6% 83.5% (many diffs)
22 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498757.3 79.6% 83.5% (many diffs)
23 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498758.3 79.6% 83.5% (many diffs)
24 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498759.3 79.6% 83.5% (many diffs)
25 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498760.3 79.6% 83.5% (many diffs)
26 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271467.1 79.4% 83.2% (many diffs)
27 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_207669.2 79.4% 83.2% (many diffs)
28 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498761.3 79.4% 83.2% (many diffs)
29 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498755.1 78.1% 81.8% (many diffs)
30 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498756.1 77.8% 81.6% (many diffs)
31 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498764.3 75.9% 79.8% (many diffs)
32 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_017315588.1 63.8% 67% (many diffs)
33 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271492.1 60.3% 63.8% (many diffs)
34 mouse 14391 Gabpb1 GA repeat binding protein, ... NR_073183.1 45.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1146
ORF length:
1080
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc cctggtagat ttgggaaaga agcttttaga agcggcacga gcaggtcaag
121atgatgaagt tcgtattttg atggcaaatg gagctccctt tactacagac tggctgggaa
181cttctccact tcatctagca gcacagtatg gtcattattc caccacagag gtactgctgc
241gagctggtgt gagcagagat gccagaacca aagtggaccg aacaccatta catatggcag
301cttctgaggg ccatgccagc atagtagagg ttttacttaa gcatggtgct gatgtcaatg
361caaaggacat gttaaagatg acagctctcc attgggccac agaacacaat catcaagagg
421tggtggaact tttaatcaaa tatggtgctg atgtacacac gcaaagtaaa ttttgtaaaa
481ctgcatttga tatttcaata gacaatggaa atgaagattt agcagagata ttacagattg
541ctatgcagaa ccaaatcaac acaaacccag agagtcctga cactgtgaca atacatgctg
601caacaccaca gtttatcatt ggacctggag gggtggtgaa cctaacaggt ctggtatctt
661cagaaaattc atccaaggca acagatgaaa cgggtgtatc tgctgttcag tttggaaact
721cttctacatc agtattagct acattagctg ccttagctga agcatctgct ccattgtcca
781attcTTCAGA AACTCCAGTA GTGGCCACAG AAGAAGTAGT TACTGCAGAA TCTGTGGATG
841GTGCCATTCA GCAAGTAGTT AGTTCAGGGG GTCAGCAAGT CATCACAATA GTTACAGATG
901GAATTCAGCT TGGAAATTTG CACTCTATTC CAACCAGTGG AATTGGTCAG CCCATCATTG
961TGACCATGCC AGATGGACAA CAAGTATTAA CAGTACCAGC AACAGACATT GCTGAAGAAA
1021CTGTTATAAG TGAAGAACCA CCAGCTAAGA GACAATGTAT CGAAATAATT GAAAACCGGG
1081TGGAATCTGC AGAAATAGAA GTAAGGAGTC TTTTACCCGG TGTGCTTTGC CGCAGTCATC
1141CAAAATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1201TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1261CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCAGAACGA TTTCGCCTGC AAACGACGCG
1321TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)