Construct: ORF TRCN0000470577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017734.1_s317c1
- Derived from:
- ccsbBroadEn_13689
- DNA Barcode:
- TGGACCGTGATACTAATAGAACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK4 (440275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470577
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440275 | EIF2AK4 | eukaryotic translation init... | NM_001013703.4 | 37.1% | 36.9% | (many diffs) |
| 2 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | NM_013719.3 | 31.9% | 32.8% | (many diffs) |
| 3 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_017318909.1 | 30.4% | 31.2% | (many diffs) |
| 4 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | NM_001177806.1 | 26.1% | 26.7% | (many diffs) |
| 5 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_006499625.3 | 26.1% | 26.7% | (many diffs) |
| 6 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_006499626.3 | 25.3% | 26% | (many diffs) |
| 7 | mouse | 27103 | Eif2ak4 | eukaryotic translation init... | XM_006499627.3 | 23.8% | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgggggccgt ggggcccccg ggcgcggccg ggacgagcct ccggagagct 121 acccgcaacg acaggaccac gagctacagg ccctggaggc catctacggc gcggacttcc 181 aagacctgcg gccggacgct tgcggaccgg tcaaagagcc ccctgaaatc aatttagttt 241 tgtaccctca aggcctaact ggtgaagaag tatatgtaaa agtggatttg agggttaaat 301 gcccacctac ctatccagat gtagttcctg aaatagagtt aaaaaatgcc aaaggtctat 361 caaatgaaag tgtcaatttg ttaaaatctc gcctagaaga actggccaag aaacactgtg 421 gggaggtgat gatctttgaa ctggcttacc acgtgcagtc atttctcagc gagcataaca 481 agccccctcc caagtctttt catgaagaaa tgctggaaag gcgggctcag gaggagcagc 541 agaggctgtt ggaggccaag cggaaagaag agcaggagca acgtgaaatc ctgcatgaga 601 ttcagagaag gaaagaagag ataaaagaag agaaaaaaag gaaagaaatg gctaagcagg 661 aacgtttgga aattgctagt ttgtcaaacc aagatcatac ctctaagaag gacccaggag 721 gacacagaac ggctgccatt ctacatggag gctctcctga ctttgtagga aatggtaaac 781 atcgggcaaa ctcctcagga aggtctaggc gagaacgtca gtattctgta tgtaatagtg 841 aagattctcc tggctcttgt gaaattctgt atttcaatat ggggagtcct gatcagctca 901 tggtgcacaa agggaaatgt attggcagtg atgaacaact tggaaaatta gtctacaatg 961 ctttggaaac agccactggt ggctttgtct tgttgtatga gtgggtcctt cagtggcaga 1021 aaaaaatggg tccattcctt accagtcaag aaaaagagaa gattgataag tgcaaaaagc 1081 agattcaagg aacagaaaca gaattcaact cactggtaaa attgagccat ccaaatgtag 1141 tacgctacct tgcaatgaat ctcaaagagc aagacgactc catcgtggtg gacattttag 1201 tggagcacat tagtggggtc tctcttgctg cacacctgag ccactcaggc cccatccctg 1261 tgcatcagct tcgcaggtac acagctcagc tcctgtcagg ccttgattat ctgcacagca 1321 attctgtggt gcataaggtc ctgagtgcat ctaatgtctt ggtggatgca gaaggcaccg 1381 tcaagattac ggactatagc atttctaagc gcctcgcaga catttgcaag gaggatgtgt 1441 ttgagcaaac ccgagttcgt tttagtgaca atgctctgcc ttataaaacg ggGAAGAAAG 1501 GAGATGTTTG GCGTCTTGGC CTTCTGCTGC TGTCCCTCAG CCAAGGACAG GAATGTGGAG 1561 AGTACCCTGT GACCATCCCT AGTGACTTAC CAGCTGACTT TCAAGATTTT CTAAAGAAAT 1621 GTGTGTGCTT GGATGACAAG GAAAGATGGA GTCCCCAGCA GTTGTTGAAA CACAGCTTTA 1681 TAAATCCCCA GCCAAAAATG CCTCTAGTGG AACAAAGTCC TGAAGATTCT GAAGGACAAG 1741 ATTATGTTGA GACTGTTATT CCTAGCAACC GGCTACCCAG TGCTGCCTTC TTTAGTGAGA 1801 CACAGAGACA GTTTTCCCGA TACTTCATTG AGTTTGAAGA ATTACAACTT CTTGGTAAAG 1861 GAGCTTTTGG AGCTGTCATC AAGGTGTGGT ACAGAGTCAT TCCCAGTCCC CTTTACCCAA 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAT GGACCGTGAT ACTAATAGAA CGAACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att