Construct: ORF TRCN0000470587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011616.1_s317c1
- Derived from:
- ccsbBroadEn_06012
- DNA Barcode:
- GCCAACCATGCCTCGCACACACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPP1 (1200)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470587
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1200 | TPP1 | tripeptidyl peptidase 1 | NM_000391.4 | 99.9% | 99.8% | 1117C>G |
| 2 | mouse | 12751 | Tpp1 | tripeptidyl peptidase I | NM_009906.6 | 86.2% | 88.2% | (many diffs) |
| 3 | mouse | 12751 | Tpp1 | tripeptidyl peptidase I | XM_011241668.2 | 74% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg actccaagcc tgcctcctag ggctctttgc cctcatcctc tctggcaaat 121 gcagttacag cccggagccc gaccagcgga ggacgctgcc cccaggctgg gtgtccctgg 181 gccgtgcgga ccctgaggaa gagctgagtc tcacctttgc cctgagacag cagaatgtgg 241 aaagactctc ggagctggtg caggctgtgt cggatcccag ctctcctcaa tacggaaaat 301 acctgaccct agagaatgtg gctgatctgg tgaggccatc cccactgacc ctccacacgg 361 tgcaaaaatg gctcttggca gccggagccc agaagtgcca ttctgtgatc acacaggact 421 ttctgacttg ctggctgagc atccgacaag cagagctgct gctccctggg gctgagtttc 481 atcactatgt gggaggacct acggaaaccc atgttgtaag gtccccacat ccctaccagc 541 ttccacaggc cttggccccc catgtggact ttgtgggggg actgcaccgt tttcccccaa 601 catcatccct gaggcaacgt cctgagccgc aggtgacagg gactgtaggc ctgcatctgg 661 gggtaacccc ctctgtgatc cgtaagcgat acaacttgac ctcacaagac gtgggctctg 721 gcaccagcaa taacagccaa gcctgtgccc agttcctgga gcagtatttc catgactcag 781 acctggctca gttcatgcgc ctcttcggtg gcaactttgc acatcaggca tcagtagccc 841 gtgtggttgg acaacagggc cggggccggg ccgggattga ggccagtcta gatgtgcagt 901 acctgatgag tgctggtgcc aacatctcca cctgggtcta cagtagccct ggccggcatg 961 agggacagga gcccttcctg cagtggctca tgctgctcag taatgagtca gccctgccac 1021 atgtgcatac tgtgagctat ggagatgatg aggactccct cagcagcgcc tacatccagc 1081 gggtcaacac tgagctcatg aaggctgccg ctcggggtct caccctgctc ttcgcctcag 1141 gtgacagtgg ggccgggtgt tggtctgtct ctggaagaca cgagttccgc cctaccttcc 1201 ctgcctccag cccctatgtc accacagtgg gaggcacatc cttccaggaa cctttcctca 1261 tcacaaatga aattgttgac tatatcagtg gtggtggctt cagcaatgtg ttccCACGGC 1321 CTTCATACCA GGAGGAAGCT GTAACGAAGT TCCTGAGCTC TAGCCCCCAC CTGCCACCAT 1381 CCAGTTACTT CAATGCCAGT GGCCGTGCCT ACCCAGATGT GGCTGCACTT TCTGATGGCT 1441 ACTGGGTGGT CAGCAACAGA GTGCCCATTC CATGGGTGTC CGGAACCTCG GCCTCTACTC 1501 CAGTGTTTGG GGGGATCCTA TCCTTGATCA ATGAGCACAG GATCCTTAGT GGCCGCCCCC 1561 CTCTTGGCTT TCTCAACCCA AGGCTCTACC AGCAGCATGG GGCAGGACTC TTTGATGTAA 1621 CCCGTGGCTG CCATGAGTCC TGTCTGGATG AAGAGGTAGA GGGCCAGGGT TTCTGCTCTG 1681 GTCCTGGCTG GGATCCTGTA ACAGGCTGGG GAACACCCAA CTTCCCAGCT TTGCTGAAGA 1741 CTCTACTCAA CCCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCCAACCATG CCTCGCACAC 1921 ACCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt