Construct: ORF TRCN0000470601
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007249.1_s317c1
- Derived from:
- ccsbBroadEn_02040
- DNA Barcode:
- GAATTGAGCCACAAATCTGGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAE1 (8883)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470601
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8883 | NAE1 | NEDD8 activating enzyme E1 ... | NM_003905.4 | 100% | 100% | |
| 2 | human | 8883 | NAE1 | NEDD8 activating enzyme E1 ... | NM_001286500.1 | 99.4% | 99.4% | 156_164delAGGTAACGT |
| 3 | human | 8883 | NAE1 | NEDD8 activating enzyme E1 ... | NM_001018159.1 | 97.9% | 97% | (many diffs) |
| 4 | human | 8883 | NAE1 | NEDD8 activating enzyme E1 ... | NM_001018160.1 | 83.3% | 83.3% | 0_1ins267 |
| 5 | human | 8883 | NAE1 | NEDD8 activating enzyme E1 ... | XM_005256215.1 | 83.3% | 83.3% | 0_1ins267 |
| 6 | mouse | 234664 | Nae1 | NEDD8 activating enzyme E1 ... | NM_144931.3 | 92.5% | 97% | (many diffs) |
| 7 | mouse | 234664 | Nae1 | NEDD8 activating enzyme E1 ... | XM_006530884.2 | 88.1% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1668
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagctggga aagctgctca aggagcagaa gtacgaccgg cagctgaggt 121 tgtggggtga tcatgggcaa gaggctttag aatctgctca tgtttgccta ataaatgcaa 181 cagccacagg aactgaaatt cttaaaaact tggtactacc aggtattggt tcgtttacaa 241 ttattgatgg aaatcaggtc agcggagaag atgctggaaa caatttcttc cttcaaagaa 301 gcagtatcgg caagaaccga gctgaagctg ccatggaatt cttacaagaa ttaaatagcg 361 atgtctctgg aagttttgtg gaagagagtc cagaaaacct tctagacaat gatccctcat 421 ttttctgtag gtttactgtt gtagttgcaa ctcagcttcc tgaaagcact tcactacgct 481 tagcagatgt cctctggaat tcccagattc ctcttttgat ctgtaggaca tatggactag 541 ttggttatat gaggatcatt ataaaagaac atccagtaat agaatctcat ccagataatg 601 cattagagga tctacgacta gataagccat ttcctgaact gagagaacat tttcagtcct 661 atgatttgga tcatatggaa aaaaaggacc acagtcatac tccatggatt gtgatcatag 721 ctaaatattt agcacagtgg tatagtgaaa caaatggacg aatacctaaa acgtataaag 781 aaaaagagga cttcagagat ttgattagac aaggaattct aaaaaatgaa aatggggctc 841 cagaagatga agagaatttt gaagaagcta ttaaaaatgt gaacacagca ctaaatacaa 901 ctcagatccc aagcagtatt gaagatatat ttaatgatga tcgctgcata aatatcacca 961 aacagactcc atcattttgg attttagctc gtgccttaaa ggaatttgtg gccaaagagg 1021 gtcaaggaaa tttacctgtt cgaggcacaa ttcctgatat gattgcagat tcaggcaaat 1081 atataaaact gcaaaacgtt taccgtgaaa aagcaaagaa agatgctgcc gctgtgggta 1141 atcatgttgc caaattgctg cagtccattg gccaggcacc agagtccatt tcagagaaag 1201 aattaaaatt actctgcagc aattctgcat ttcttcgagt ggtaagatgt cgatccttag 1261 ctgaagaata tggtttggat acaattaaca aggatgaaat tatttctagc atggacaatc 1321 cagataatga aatagtgttg tacttaatgt tacgggctgt tgatagattt cataaaCAAC 1381 AGGGTAGATA TCCAGGAGTA TCTAACTATC AAGTTGAAGA AGATATAGGA AAGTTGAAGT 1441 CTTGTCTCAC TGGCTTCCTT CAGGAATATG GTTTATCTGT AATGGTGAAA GATGATTATG 1501 TCCACGAATT TTGCCGATAT GGAGCTGCTG AGCCACATAC CATTGCTGCA TTCTTGGGGG 1561 GAGCTGCTGC TCAAGAGGTC ATCAAAATAA TCACCAAACA ATTTGTAATT TTTAATAATA 1621 CTTACATTTA CAGTGGCATG TCACAAACTT CAGCAACTTT CCAGTTGTAC CCAACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGAGAATTGA GCCACAAATC TGGGACAACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt