Construct: ORF TRCN0000470610
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015207.1_s317c1
- Derived from:
- ccsbBroadEn_05252
- DNA Barcode:
- CTAATGAAATACCCCCTTTCCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPNE2 (221184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470610
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221184 | CPNE2 | copine 2 | NM_152727.6 | 100% | 100% | |
| 2 | human | 221184 | CPNE2 | copine 2 | XR_001751870.1 | 69.1% | 1_228del;1395_1396ins134;1739_2049del | |
| 3 | mouse | 234577 | Cpne2 | copine II | NM_153507.2 | 88.6% | 94.5% | (many diffs) |
| 4 | mouse | 234577 | Cpne2 | copine II | XM_017312732.1 | 57.5% | 59.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1710
- ORF length:
- 1644
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccacataccc agtgggggtg ccccagcagc gggggcagcc cccatgggcc 121 cccagtattg cgtgtgcaag gtggagctgt cagtgagtgg ccagaaccta ctggaccggg 181 atgttacctc caagtccgac cccttctgtg tcctctttac agagaacaat ggcagatgga 241 tcgagtacga caggacagaa accgcgatca acaacctcaa ccccgccttc tccaagaagt 301 tcgtgcttga ctaccacttc gaggaggtac agaagctcaa gttcgcgctc tttgaccagg 361 acaagtccag tatgcggctg gacgagcatg acttcctggg ccagttctcc tgcagcctgg 421 gcacgatcgt ctccagcaag aagatcacta ggcctctgct gctgctgaat gacaagcctg 481 cggggaaggg cttgattacg atcgctgccc aggagctgtc cgacaaccgc gtcatcacac 541 taagcctggc gggcaggagg ctggacaaga aggacctctt tgggaagtca gacccctttc 601 tggagtttta taagccagga gacgatggca agtggatgct ggtccacagg actgaggtga 661 tcaagtacac actggaccct gtgtggaagc cattcacagt gcccttggtg tccctgtgtg 721 atggggacat ggagaagccc atccaggtca tgtgctacga ctatgacaat gacgggggcc 781 atgacttcat cggcgagttc cagacctcag tgtcacagat gtgtgaggct cgagacagcg 841 tcccgctgga gttcgagtgc atcaacccca agaagcagag gaagaagaag aactataaaa 901 actcgggcat catcatcctg cgatcctgca agataaaccg agactactcc ttccttgact 961 acatcctggg aggctgccag ctcatgttca ccgttggaat agactttaca gcctccaacg 1021 ggaatcccct cgacccttcc tctttgcact atatcaaccc tatgggcacc aacgaatatc 1081 tgtcggccat ctgggctgtt gggcagatca ttcaggacta cgacagtgat aagatgtttc 1141 cagctctggg attcggggcc cagttacccc cagactggaa ggtctcccat gagtttgcca 1201 tcaacttcaa ccccaccaac cccttctgct caggtgtgga tggtattgcc caggcgtact 1261 cagcttgcct gccccacatc cgcttctacg gtcctaccaa tttcTCCCCC ATCGTCAACC 1321 ACGTGGCCCG GTTTGCGGCC CAGGCCACAC AACAGCGGAC GGCCACGCAG TACTTCATCC 1381 TCCTCATCAT CACGGACGGG GTCATCAGTG ACATGGAGGA GACACGGCAT GCCGTGGTGC 1441 AGGCTTCCAA GCTGCCCATG TCCATCATCA TCGTGGGCGT GGGCAATGCG GACTTCGCTG 1501 CCATGGAGTT CCTGGATGGG GACAGCCGCA TGCTGCGCTC CCACACGGGG GAGGAGGCAG 1561 CCCGCGATAT TGTGCAGTTC GTTCCCTTTC GAGAGTTCCG CAACGCAGCA AAAGAGACCT 1621 TGGCCAAAGC TGTGCTGGCG GAGCTGCCCC AACAAGTTGT GCAGTATTTC AAGCATAAAA 1681 ACCTGCCCCC CACCAACTCG GAGCCCGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1741 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1801 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTAAT 1861 GAAATACCCC CTTTCCAAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1921 tgaaagatt