Transcript: Mouse XM_017312732.1

PREDICTED: Mus musculus copine II (Cpne2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cpne2 (234577)
Length:
1670
CDS:
528..1604

Additional Resources:

NCBI RefSeq record:
XM_017312732.1
NBCI Gene record:
Cpne2 (234577)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017312732.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000366085 TGCTACGACTATGACAGTAAT pLKO_005 1215 CDS 100% 13.200 18.480 N Cpne2 n/a
2 TRCN0000379079 AGCTCAAGTTCGCCCTGTTTG pLKO_005 796 CDS 100% 10.800 15.120 N Cpne2 n/a
3 TRCN0000366008 ATACACCGAAACTACTCATTC pLKO_005 1395 CDS 100% 10.800 15.120 N Cpne2 n/a
4 TRCN0000111652 GCATCATTATTCTGAGATCAT pLKO.1 1369 CDS 100% 4.950 6.930 N Cpne2 n/a
5 TRCN0000111654 CCAGGTTATGTGCTACGACTA pLKO.1 1205 CDS 100% 4.050 5.670 N Cpne2 n/a
6 TRCN0000366083 CATAGGACCGAGGTGATTAAG pLKO_005 1107 CDS 100% 13.200 10.560 N Cpne2 n/a
7 TRCN0000379150 CCAGTTGGATGAGCATGATTT pLKO_005 836 CDS 100% 13.200 9.240 N Cpne2 n/a
8 TRCN0000111651 CGACCCTTCTTCTCTCCATTA pLKO.1 1493 CDS 100% 10.800 7.560 N Cpne2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017312732.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05252 pDONR223 100% 57.5% 59.6% None (many diffs) n/a
2 ccsbBroad304_05252 pLX_304 0% 57.5% 59.6% V5 (many diffs) n/a
3 TRCN0000470610 CTAATGAAATACCCCCTTTCCAAG pLX_317 25% 57.5% 59.6% V5 (many diffs) n/a
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