Construct: ORF TRCN0000470617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011448.1_s317c1
- Derived from:
- ccsbBroadEn_07883
- DNA Barcode:
- TAGCAAGCAGGAAAACACGAAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC7A8 (23428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_012244.4 | 99.8% | 100% | 1146C>T;1170T>C |
| 2 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_001267036.1 | 76.4% | 71.7% | (many diffs) |
| 3 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_182728.3 | 61.9% | 62% | 0_1ins609;537C>T;561T>C |
| 4 | human | 23428 | SLC7A8 | solute carrier family 7 mem... | NM_001267037.1 | 58% | 52.2% | (many diffs) |
| 5 | mouse | 50934 | Slc7a8 | solute carrier family 7 (ca... | NM_016972.2 | 88.7% | 91.9% | (many diffs) |
| 6 | mouse | 50934 | Slc7a8 | solute carrier family 7 (ca... | XM_006519250.2 | 55.2% | 56.4% | (many diffs) |
| 7 | mouse | 50934 | Slc7a8 | solute carrier family 7 (ca... | XM_011245114.1 | 51.7% | 45.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1671
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaaggagcc aggcaccgaa acaacaccga aaagaaacac ccaggtgggg 121 gcgagtcgga cgccagcccc gaggctggtt ccggaggggg cggagtagcc ctgaagaaag 181 agatcggatt ggtcagtgcc tgtggtatca tcgtagggaa catcatcggc tctggaatct 241 ttgtctcgcc aaagggagtg ctggagaatg ctggttctgt gggccttgct ctcatcgtct 301 ggattgtgac gggcttcatc acagttgtgg gagccctctg ctatgctgaa ctcggggtca 361 ccatccccaa atctggaggt gactactcct atgtcaagga catcttcgga ggactggctg 421 ggttcctgag gctgtggatt gctgtgctgg tgatctaccc caccaaccag gctgtcatcg 481 ccctcacctt ctccaactac gtgctgcagc cgctcttccc cacctgcttc cccccagagt 541 ctggccttcg gctcctggct gccatctgct tattgctcct cacatgggtc aactgttcca 601 gtgtgcggtg ggccacccgg gttcaagaca tcttcacagc tgggaagctc ctggccttgg 661 ccctgattat catcatgggg attgtacaga tatgcaaagg agagtacttc tggctggagc 721 caaagaatgc atttgagaat ttccaggaac ctgacatcgg cctcgtcgca ctggctttcc 781 ttcagggctc ctttgcctat ggaggctgga actttctgaa ttacgtgact gaggagcttg 841 ttgatcccta caagaacctt cccagagcca tcttcatctc catcccactg gtcacatttg 901 tgtatgtctt tgccaatgtc gcttatgtca ctgcaatgtc cccccaggag ctgctggcat 961 ccaacgccgt cgctgtgact tttggagaga agctcctagg agtcatggcc tggatcatgc 1021 ccatttctgt tgccctgtcc acatttggag gagttaatgg gtctctcttc acctcctctc 1081 ggctgttctt cgctggagcc cgagagggcc accttcccag tgtgttggcc atgatccacg 1141 tgaagcgctg caccccaatc ccagccctgc tcttcacatg catctccacc ctgctgatgc 1201 tggtcaccag tgacatgtac acactcatca actacgtggg cttcatcaac tacctcttct 1261 atggggtcac ggttgctgga cagatagtcc ttcgctggaa gaagcctgat aTCCCCCGCC 1321 CCATCAAGAT CAACCTGCTG TTCCCCATCA TCTACTTGCT GTTCTGGGCC TTCCTGCTGG 1381 TCTTCAGCCT GTGGTCAGAG CCGGTGGTGT GTGGCATTGG CCTGGCCATC ATGCTGACAG 1441 GAGTGCCTGT CTATTTCCTG GGTGTTTACT GGCAACACAA GCCCAAGTGT TTCAGTGACT 1501 TCATTGAGCT GCTAACCCTG GTGAGCCAGA AGATGTGTGT GGTCGTGTAC CCCGAGGTGG 1561 AGCGGGGCTC AGGGACAGAG GAGGCTAATG AGGACATGGA GGAGCAGCAG CAGCCCATGT 1621 ACCAACCCAC TCCCACGAAG GACAAGGACG TGGCGGGGCA GCCCCAGCCC TGCCCAACTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGATAGC AAGCAGGAAA ACACGAAGGC ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt