Transcript: Mouse XM_006519250.2

PREDICTED: Mus musculus solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Slc7a8 (50934)
Length:
2987
CDS:
119..1108

Additional Resources:

NCBI RefSeq record:
XM_006519250.2
NBCI Gene record:
Slc7a8 (50934)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006519250.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000079456 CCAATGCAGTTGCTGTGACTT pLKO.1 405 CDS 100% 4.950 3.465 N Slc7a8 n/a
2 TRCN0000079453 GCACTGACTTTGGAGACCTTA pLKO.1 2375 3UTR 100% 4.950 3.465 N Slc7a8 n/a
3 TRCN0000079454 GCCATTATGCTGACAGGAGTT pLKO.1 869 CDS 100% 4.050 2.835 N Slc7a8 n/a
4 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1182 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006519250.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07883 pDONR223 100% 55.2% 56.4% None (many diffs) n/a
2 ccsbBroad304_07883 pLX_304 0% 55.2% 56.4% V5 (many diffs) n/a
3 TRCN0000470617 TAGCAAGCAGGAAAACACGAAGGC pLX_317 22.7% 55.2% 56.4% V5 (many diffs) n/a
4 TRCN0000492299 TTCTAATTTCTGACTATTCGACAA pLX_317 13.1% 55.2% 56.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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