Construct: ORF TRCN0000470621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015259.1_s317c1
- Derived from:
- ccsbBroadEn_08566
- DNA Barcode:
- AGCCCCTATTGAGCGATGCATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5 (55695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 100% | 100% | |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 98.4% | 91.4% | 1284_1285insAGCTC;1394_1410del |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 91.9% | 91.8% | 1287_1287delGins112 |
| 4 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 85.5% | 1_36del;790_791insGGAA;1431_1626del | |
| 5 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 83.8% | 83.6% | 212_213ins114;1173_1173delGins112 |
| 6 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 81.7% | 60.7% | 754_755insGGAA;1143_1144ins251 |
| 7 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 73.8% | (many diffs) | |
| 8 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 73.8% | (many diffs) | |
| 9 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 56.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1464
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgtatgct gcagctgcag gcgtgttggc cggcgtggag agccgccagg 121 gctctatcaa ggggttggtg tactccagca acttccagaa cgtgaagcag ctgtacgcgc 181 tggtgtgcga aacgcagcgc tactccgccg tgctggatgc tgtgatcgcc agcgccggcc 241 tcctccgtgc ggagaagaag ctgcggccgc acctggccaa ggtgctagtg tatgagttgt 301 tgttgggaaa gggctttcga gggggtgggg gccgatggaa ggctctgttg ggccggcacc 361 aggcgaggct caaggctgag ttggctcggc tcaaggttca tcggggtgtg agccggaatg 421 aggacctgtt ggaagtggga tccaggcctg gtccagcctc ccagctgcct cgatttgtgc 481 gtgtgaacac tctcaagacc tgctccgatg atgtagttga ttatttcaag agacaaggtt 541 tctcctatca gggtcgggct tccagcctcg atgacttacg agccctcaag gggaagcatt 601 ttctcctgga ccccttgatg ccggagctgc tggtgtttcc cgcccagaca gatctgcatg 661 aacacccact gtaccgggcc ggacacctca ttctgcagga cagggccagc tgtctcccag 721 ccatgctgct ggaccccccg ccaggctccc atgtcatcga tgcctgtgcc gccccaggca 781 ataagaccag tcacttggct gctcttctga agaaccaagg gaagatcttt gcctttgacc 841 tggatgccaa gcggctggca tccatggcca cgctgctggc ccgggctggc gtctcttgct 901 gtgaactggc tgaggaggac ttcctggcgg tctccccctc ggatccacgc taccatgagg 961 tccactacat cctgctggat ccttcctgca gtggctcggg tatgccgagc agacagctgg 1021 aggagcccgg ggcaggcaca cctagcccgg tgcgtctgca tgccctggca gggttccagc 1081 agcgagccct gtgccacgca ctcactttcc cttccctgca gcggctcgtc tactccacgt 1141 gctcccTCTG CCAGGAGGAG AATGAAGACG TGGTGCGAGA TGCGCTGCAG CAGAACCCGG 1201 GCGCCTTCAG GCTAGCTCCC GCCCTGCCTG CCTGGCCCCA CCGAGGCCTG AGCACGTTCC 1261 CGGGTGCCGA GCACTGCCTC CGGGCCTCCC CTGAGACCAC ACTCAGCAGT GGCTTCTTCG 1321 TTGCTGTAAT TGAACGGGTC GAGGTGCCAA GCTCAGCCTC ACAGGCCAAA GCATCAGCAC 1381 CAGAACGCAC ACCCAGCCCA GCCCCAAAGA GAAAGAAGAG ACAGCAAAGA GCCGCAGCCG 1441 GTGCTTGCAC ACCGCCTTGC ACATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCCCCTATTG 1621 AGCGATGCAT TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att