Construct: ORF TRCN0000470625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004076.1_s317c1
- Derived from:
- ccsbBroadEn_03995
- DNA Barcode:
- ATCGGGCATCATCTCAAAGATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN4R (65078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470625
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65078 | RTN4R | reticulon 4 receptor | NM_023004.6 | 100% | 100% | |
| 2 | mouse | 65079 | Rtn4r | reticulon 4 receptor | NM_022982.2 | 83.1% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagggcgtcc gctggaggga gccggctgct ggcatgggtg ctgtggctgc 121 aggcctggca ggtggcagcc ccatgcccag gtgcctgcgt atgctacaat gagcccaagg 181 tgacgacaag ctgcccccag cagggcctgc aggctgtgcc cgtgggcatc cctgctgcca 241 gccagcgcat cttcctgcac ggcaaccgca tctcgcatgt gccagctgcc agcttccgtg 301 cctgccgcaa cctcaccatc ctgtggctgc actcgaatgt gctggcccga attgatgcgg 361 ctgccttcac tggcctggcc ctcctggagc agctggacct cagcgataat gcacagctcc 421 ggtctgtgga ccctgccaca ttccacggcc tgggccgcct acacacgctg cacctggacc 481 gctgcggcct gcaggagctg ggcccggggc tgttccgcgg cctggctgcc ctgcagtacc 541 tctacctgca ggacaacgcg ctgcaggcac tgcctgatga caccttccgc gacctgggca 601 acctcacaca cctcttcctg cacggcaacc gcatctccag cgtgcccgag cgcgccttcc 661 gtgggctgca cagcctcgac cgtctcctac tgcaccagaa ccgcgtggcc catgtgcacc 721 cgcatgcctt ccgtgacctt ggccgcctca tgacactcta tctgtttgcc aacaatctat 781 cagcgctgcc cactgaggcc ctggcccccc tgcgtgccct gcagtacctg aggctcaacg 841 acaacccctg ggtgtgtgac tgccgggcac gcccactctg ggcctggctg cagaagttcc 901 gcggctcctc ctccgaggtg ccctgcagcc tcccgcaacg cctggctggc cgtgacctca 961 aacgcctagc tgccaatgac ctgcagggct gcgctgtggc caccggccct taccatccca 1021 tctggaccgg cagggCCACC GATGAGGAGC CGCTGGGGCT TCCCAAGTGC TGCCAGCCAG 1081 ATGCCGCTGA CAAGGCCTCA GTACTGGAGC CTGGAAGACC AGCTTCGGCA GGCAATGCGC 1141 TGAAGGGACG CGTGCCGCCC GGTGACAGCC CGCCGGGCAA CGGCTCTGGC CCACGGCACA 1201 TCAATGACTC ACCCTTTGGG ACTCTGCCTG GCTCTGCTGA GCCCCCGCTC ACTGCAGTGC 1261 GGCCCGAGGG CTCCGAGCCA CCAGGGTTCC CCACCTCGGG CCCTCGCCGG AGGCCAGGCT 1321 GTTCACGCAA GAACCGCACC CGCAGCCACT GCCGTCTGGG CCAGGCAGGC AGCGGGGGTG 1381 GCGGGACTGG TGACTCAGAA GGCTCAGGTG CCCTACCCAG CCTCACCTGC AGCCTCACCC 1441 CCCTGGGCCT GGCGCTGGTG CTGTGGACAG TGCTTGGGCC CTGCTGCCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA ATCGGGCATC ATCTCAAAGA TCCCACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt