Construct: ORF TRCN0000470637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010662.1_s317c1
- Derived from:
- ccsbBroadEn_03909
- DNA Barcode:
- TGGGAGCCGTAGTCCGCCAAAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS14 (63931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470637
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63931 | MRPS14 | mitochondrial ribosomal pro... | NM_022100.3 | 100% | 100% | |
| 2 | human | 63931 | MRPS14 | mitochondrial ribosomal pro... | NR_037606.1 | 17% | 1_47del;92_183del;524_2252del | |
| 3 | mouse | 64659 | Mrps14 | mitochondrial ribosomal pro... | NM_025474.4 | 86.4% | 86.7% | (many diffs) |
| 4 | mouse | 64659 | Mrps14 | mitochondrial ribosomal pro... | NM_001347121.1 | 79.8% | 76.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 474
- ORF length:
- 384
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGGCCTT CATGCTGGGC TCGCTGCTGC 121 GGACGTTCAA GCAGATGGTT CCTTCATCAG CTTCAGGCCA AGTTCGAAGT CACTATGTAG 181 ACTGGAGAAT GTGGCGCGAT GTGAAGAGAC GAAAAATGGC CTATGAATAC GCAGATGAGA 241 GGCTACGTAT TAATTCACTC AGGAAGAATA CCATTTTGCC AAAAATTCTT CAGGATGTGG 301 CTGATGAAGA AATTGCTGCC CTCCCCCGGG ATAGCTGTCC TGTTAGAATC AGAAATCGGT 361 GTGTTATGAC GTCCCGTCCG CGTGGTGTGA AGCGGCGCTG GAGGCTTAGT CGTATAGTCT 421 TCCGTCACTT AGCTGACCAT GGGCAACTTT CTGGGATCCA GCGAGCGACA TGGTACCCAG 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAT GGGAGCCGTA GTCCGCCAAA GGCACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att