Construct: ORF TRCN0000470639
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004218.1_s317c1
- Derived from:
- ccsbBroadEn_09327
- DNA Barcode:
- TTCGAACATTTGCTGCTGACCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMD4 (91937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470639
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91937 | TIMD4 | T cell immunoglobulin and m... | NM_138379.3 | 99.9% | 99.7% | 719T>C |
| 2 | human | 91937 | TIMD4 | T cell immunoglobulin and m... | NM_001146726.2 | 92.5% | 92.3% | 719T>C;758_759ins84 |
| 3 | human | 91937 | TIMD4 | T cell immunoglobulin and m... | XM_017010021.2 | 85.4% | 85.4% | 677_678ins165 |
| 4 | human | 91937 | TIMD4 | T cell immunoglobulin and m... | XM_011534694.3 | 77.3% | 74.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1200
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caaagaacct ctcattctct ggctgatgat tgagttttgg tggctttacc 121 tgacaccagt cacttcagag actgttgtga cggaggtttt gggtcaccgg gtgactttgc 181 cctgtctgta ctcatcctgg tctcacaaca gcaacagcat gtgctggggg aaagaccagt 241 gcccctactc cggttgcaag gaggcgctca tccgcactga tggaatgagg gtgacctcaa 301 gaaagtcagc aaaatataga cttcagggga ctatcccgag aggtgatgtc tccttgacca 361 tcttaaaccc cagtgaaagt gacagcggtg tgtactgctg ccgcatagaa gtgcctggct 421 ggttcaacga tgtaaagata aacgtgcgcc tgaatctaca gagagcctca acaaccacgc 481 acagaacagc aaccaccacc acacgcagaa caacaacaac aagccccacc accacccgac 541 aaatgacaac aaccccagct gcacttccaa caacagtcgt gaccacaccc gatctcacaa 601 ccggaacacc actccagatg acaaccattg ccgtcttcac aacagcaaac acgtgccttt 661 cactaacccc aagcaccctt ccggaggaag ccacaggtct tctgactccc gagccTTCTA 721 AGGAAGGGCC CATCCTCACT GCAGAATCAG AAACTGTCCT CCCCAGTGAT TCCTGGAGTA 781 GTGCTGAGTC TACTTCTGCT GACACTGTCC TGCTGACATC CAAAGAGTCC AAAGTTTGGG 841 ATCTCCCATC AACATCCCAC GTGTCAATGT GGAAAACGAG TGATTCTGTG TCTTCTCCTC 901 AGCCTGGAGC ATCTGATACA GCAGTTCCTG AGCAGAACAA AACAACAAAA ACAGGACAGA 961 TGGATGGAAT ACCCATGTCA ATGAAGAATG AAATGCCCAT CTCCCAACTA CTGATGATCA 1021 TCGCCCCCTC CTTGGGATTT GTGCTCTTCG CATTGTTTGT GGCGTTTCTC CTGAGAGGGA 1081 AACTCATGGA AACCTATTGT TCGCAGAAAC ACACAAGGCT AGACTACATT GGAGATAGTA 1141 AAAATGTCCT CAATGACGTG CAGCATGGAA GGGAAGACGA AGACGGCCTT TTTACCCTCT 1201 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGATTCGA ACATTTGCTG CTGACCCGCA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt