Construct: ORF TRCN0000470662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005851.1_s317c1
- Derived from:
- ccsbBroadEn_15735
- DNA Barcode:
- TGTCAAACGCATTTCTTCACGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STARD3 (10948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470662
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10948 | STARD3 | StAR related lipid transfer... | NM_006804.4 | 99.9% | 99.7% | 647G>C |
| 2 | human | 10948 | STARD3 | StAR related lipid transfer... | XM_017024041.2 | 99.9% | 99.7% | 647G>C |
| 3 | human | 10948 | STARD3 | StAR related lipid transfer... | NM_001165937.1 | 97.9% | 86.8% | 361_362insGGTGGGTGATTGC;535_547del;647G>C |
| 4 | human | 10948 | STARD3 | StAR related lipid transfer... | NM_001165938.1 | 95.8% | 95.7% | 374_375ins54;593G>C |
| 5 | human | 10948 | STARD3 | StAR related lipid transfer... | XM_024450549.1 | 68.6% | 67.8% | (many diffs) |
| 6 | mouse | 59045 | Stard3 | START domain containing 3 | NM_021547.3 | 88.2% | 93.2% | (many diffs) |
| 7 | mouse | 59045 | Stard3 | START domain containing 3 | XM_006533896.3 | 88.2% | 93.2% | (many diffs) |
| 8 | mouse | 59045 | Stard3 | START domain containing 3 | XM_006533894.1 | 82.5% | 79.8% | (many diffs) |
| 9 | mouse | 59045 | Stard3 | START domain containing 3 | XM_006533895.3 | 82.5% | 79.8% | (many diffs) |
| 10 | mouse | 59045 | Stard3 | START domain containing 3 | XM_006533897.3 | 77.9% | 80.8% | (many diffs) |
| 11 | mouse | 59045 | Stard3 | START domain containing 3 | XR_388504.1 | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caagctgccc agggagctga cccgagactt ggagcgcagc ctgcctgccg 121 tggcctccct gggctcctca ctgtcccaca gccagagcct ctcctcgcac ctccttccgc 181 cgcctgagaa gcgaagggcc atctctgatg tccgccgcac cttctgtctc ttcgtcacct 241 tcgacctgct cttcatctcc ctgctctgga tcatcgaact gaataccaac acaggcatcc 301 gtaagaactt ggagcaggag atcatccagt acaactttaa aacttccttc ttcgacatct 361 ttgtcctggc cttcttccgc ttctctggac tgctcctagg ctatgccgtg ctgcggctcc 421 ggcactggtg ggtgattgcg gtcacgacgc tggtgtccag tgcattcctc attgtcaagg 481 tcatcctctc tgagctgctc agcaaagggg catttggcta cctgctcccc atcgtctctt 541 ttgtcctcgc ctggttggag acctggttcc ttgacttcaa agtcctaccc caggaagctg 601 aagaggagcg atggtatctt gccgcccagg ttgctgttgc ccgtggaccc ctgctgttct 661 ccggtgctct gtccgaggga cagttctatt cacccccaga atcctttgca gcgtctgaca 721 atgaatcaga tgaagaagtt gctgggaaga aaagtttctc tgctcaggag cgggagtaca 781 tccgccaggg gaaggaggcc acggcagtgg tggaccagat cttggcccag gaagagaact 841 ggaagtttga gaagaataat gaatatgggg acaccgtgta caccattgaa gttccctttc 901 acggcaagac gtttatcctg aagaccttcc tgccctgtcc tgcggagctc gtgtaccagg 961 aggtgatccT GCAGCCCGAG AGGATGGTGC TGTGGAACAA GACAGTGACT GCCTGCCAGA 1021 TCCTGCAGCG AGTGGAAGAC AACACCCTCA TCTCCTATGA CGTGTCTGCA GGGGCTGCGG 1081 GCGGCGTGGT CTCCCCAAGG GACTTCGTGA ATGTCCGGCG CATTGAGCGG CGCAGGGACC 1141 GATACTTGTC ATCAGGGATC GCCACCTCAC ACAGTGCCAA GCCCCCGACG CACAAATATG 1201 TCCGGGGAGA GAATGGCCCT GGGGGCTTCA TCGTGCTCAA GTCGGCCAGT AACCCCCGTG 1261 TTTGCACCTT TGTCTGGATT CTTAATACAG ATCTCAAGGG CCGCCTGCCC CGGTACCTCA 1321 TCCACCAGAG CCTCGCGGCC ACCATGTTTG AATTTGCCTT TCACCTGCGA CAGCGCATCA 1381 GCGAGCTGGG GGCCCGGGCG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1441 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1501 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGTC AAACGCATTT 1561 CTTCACGCTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt