Construct: ORF TRCN0000470687

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011167.1_s317c1
Derived from:
ccsbBroadEn_12538
DNA Barcode:
TTAGGGATGGAAAGTCCTTAGTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRPM8 (79054)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470687
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79054 TRPM8 transient receptor potentia... XM_024453134.1 75.6% 75.6% 0_1ins249;838_1167del
2 human 79054 TRPM8 transient receptor potentia... XM_024453133.1 73.6% 72.2% (many diffs)
3 human 79054 TRPM8 transient receptor potentia... XM_024453132.1 66.3% 66.2% (many diffs)
4 human 79054 TRPM8 transient receptor potentia... XM_017004891.1 64.9% 63.7% (many diffs)
5 human 79054 TRPM8 transient receptor potentia... NM_024080.5 61.6% 61.5% (many diffs)
6 human 79054 TRPM8 transient receptor potentia... XM_011511810.2 60.4% 59.3% (many diffs)
7 mouse 171382 Trpm8 transient receptor potentia... NM_134252.3 54.3% 58% (many diffs)
8 mouse 171382 Trpm8 transient receptor potentia... XM_006529225.1 54.3% 58% (many diffs)
9 mouse 171382 Trpm8 transient receptor potentia... XM_006529226.1 54.3% 58% (many diffs)
10 mouse 171382 Trpm8 transient receptor potentia... XM_006529229.1 50.4% 50.8% (many diffs)
11 mouse 171382 Trpm8 transient receptor potentia... XM_017319118.1 35.7% 34.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2115
ORF length:
2046
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcaggccatc aatacctcca tcaaaaataa aattccttgt gtggtggtgg
121aaggctcggg ccagatcgct gatgtgatcg ctagcctggt ggaggtggag gatgccctga
181catcttctgc cgtcaaggag aagctggtgc gctttttacc ccgcacggtg tcccggctgc
241ctgaggagga gactgagagt tggatcaaat ggctcaaaga aattctcgaa tgttctcacc
301tattaacagt tattaaaatg gaagaagctg gggatgaaat tgtgagcaat gccatctcct
361acgctctata caaagccttc agcaccagtg agcaagacaa ggataactgg aatgggcagc
421tgaagcttct gctggagtgg aaccagctgg acttagccaa tgatgagatt ttcaccaatg
481accgccgatg ggagtctgct gaccttcaag aagtcatgtt tacggctctc ataaaggaca
541gacccaagtt tgtccgcctc tttctggaga atggcttgaa cctacggaag tttctcaccc
601atgatgtcct cactgaactc ttctccaacc acttcagcac gcttgtgtac cggaatctgc
661agatcgccaa gaattcctat aatgatgccc tcctcacgtt tgtctggaaa ctggttgcga
721acttccgaag aggcttccgg aaggaagaca gaaatggccg ggacgagatg gacatagaac
781tccacgacgt gtctcctatt actcggcacc ccctgcaagc tctcttcatc tgggccattc
841ttcagaataa gaaggaactc tccaaagtca tttgggagca gaccaggggc tgcactctgg
901cagccctggg agccagcaag cttctgaaga ctctggccaa agtgaagaac gacatcaatg
961ctgctgggga gtccgaggag ctggctaatg agtacgagac ccgggctgtt gagctgttca
1021ctgagtgtta cagcagcgat gaagacttgg cagaacagct gctggtctat tcctgtgaag
1081cttggggtgg aagcaactgt ctggagctgg cggtggaggc cacagaccag catttcatcg
1141cccagcctgg ggtccagtgg tacgtaaatg gggtgaatta ttttactgac ctgtggaatg
1201tgatggacac gctggggctt ttttacttca tagcaggaat tgtatttcgg ctccactctt
1261ctaataaaag ctctttgtat tctggacgag tcattttctg tctggactac attattttca
1321ctctaagatt gatccacatt tttactgtaa gcagaaactt aggacccaag attataatgc
1381tgcagaggat gctgatcgat gtgttcttct tcctgttcct ctttgcggtg tggatggtgg
1441cctttggcgt ggccaggcaa gggatcctta ggcagaatga gcagcgctgg aggtggatat
1501tccgttcggt catctacgag ccctacctgg ccatgttcgg ccaggtgccc agtgacgtgg
1561atggtaccac gtatgacttt gcccactgca ccttcactgg gaatgagtcc aagccactgt
1621gtgtggagct ggatgagcac aacctgcccc ggttccccga gtggatcacc atccccctgg
1681tgtgcatcta catgttatcc accaacatcc tgctggtcaa cctgctggtc gccatgtttg
1741gctacacggt gggcaccgtc caggagaaca atgaccaggt ctggaagttc cagaggtact
1801tccTGGTGCA GGAGTACTGC AGCCGCCTCA ATATCCCCTT CCCCTTCATC GTCTTCGCTT
1861ACTTCTACAT GGTGGTGAAG AAGTGCTTCA AGTGTTGCTG CAAGGAGAAA AACATGGAGT
1921CTTCTGTCTG CTGTTTCAAA AATGAAGACA ATGAGACTCT GGCATGGGAG GGTGTCATGA
1981AGGAAAACTA CCTTGTCAAG ATCAACACAA AAGCCAACGA CACCTCAGAG GAAATGAGGC
2041ATCGATTTAG ACAACTGGAT ACAAAGCTTA ATGATCTCAA GGGTCTTCTG AAAGAGATTG
2101CTAATAAAAT CAAATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2161CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2221TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTAGGGATGG AAAGTCCTTA
2281GTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)