Construct: ORF TRCN0000470700
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000470.1_s317c1
- Derived from:
- ccsbBroadEn_03090
- DNA Barcode:
- ACCTAGTCTGGCCTCACGTTAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMOD3 (29766)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470700
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29766 | TMOD3 | tropomodulin 3 | NM_014547.5 | 100% | 100% | |
| 2 | human | 29766 | TMOD3 | tropomodulin 3 | XM_017022087.1 | 100% | 100% | |
| 3 | human | 29766 | TMOD3 | tropomodulin 3 | XM_017022088.1 | 100% | 100% | |
| 4 | mouse | 50875 | Tmod3 | tropomodulin 3 | NM_016963.2 | 86.6% | 90% | (many diffs) |
| 5 | mouse | 50875 | Tmod3 | tropomodulin 3 | XM_017313458.1 | 52.9% | 53.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc actgccattc cgtaaggact tagaaaagta caaagacctt gatgaagatg 121 agctccttgg gaatctgtca gaaacagaac tgaaacaact ggaaactgtt ttggatgatc 181 ttgaccccga gaatgccctt ctgcctgcag ggttccggca gaagaaccag acatcaaagt 241 ccaccacagg gccatttgat agagagcatc tcctttcata tctggagaaa gaagcattgg 301 agcataaaga cagggaagac tatgtgccct acactggaga aaaaaaaggg aaaatattta 361 tccccaaaca gaaacctgta cagactttta cagaagaaaa agtgtctctt gatccagaat 421 tagaagaagc tttgacaagt gcttctgata cagaattgtg tgacctcgca gcaattcttg 481 ggatgcacaa tttgataacg aatacaaagt tctgtaatat aatgggaagt agtaatggtg 541 ttgaccaaga acatttttca aatgtggtca aaggtgaaaa gattcttccg gtatttgatg 601 agccaccaaa tccaaccaat gtagaagaga gtttgaagag aactaaagaa aacgatgctc 661 atcttgttga agttaatttg aataatataa agaatatccc aattccaacc ctaaaagatt 721 ttgcaaaggc tttggaaacc aacacacatg tgaaatgttt cagtcttgca gccacccGGA 781 GCAATGACCC TGTTGCTACT GCTTTTGCAG AAATGCTGAA AGTGAACAAA ACTTTGAAGA 841 GCTTAAATGT GGAGTCCAAC TTTATCACGG GAGTTGGGAT TCTGGCACTG ATTGATGCGT 901 TAAGAGATAA TGAAACCCTG GCAGAGCTCA AGATTGACAA TCAGAGGCAG CAGTTGGGGA 961 CAGCTGTAGA ATTGGAAATG GCCAAGATGC TTGAGGAAAA TACAAATATC CTTAAATTTG 1021 GATATCAGTT TACACAGCAG GGACCACGAA CCAGAGCAGC TAATGCTATA ACAAAAAACA 1081 ATGACTTAGT GCGTAAGAGA CGAGTTGAAG GAGATCACCA GTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGAACC TAGTCTGGCC TCACGTTAAG GACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t