Construct: ORF TRCN0000470705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009445.1_s317c1
- Derived from:
- ccsbBroadEn_08905
- DNA Barcode:
- TTCGAGTATTGTATCTAGCTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMKV (79012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79012 | CAMKV | CaM kinase like vesicle ass... | NM_024046.5 | 99.9% | 99.8% | 1472A>G |
| 2 | human | 79012 | CAMKV | CaM kinase like vesicle ass... | NM_001320147.2 | 93.7% | 93.6% | 1050_1051ins93;1379A>G |
| 3 | mouse | 235604 | Camkv | CaM kinase-like vesicle-ass... | NM_145621.2 | 87.8% | 92.6% | (many diffs) |
| 4 | mouse | 235604 | Camkv | CaM kinase-like vesicle-ass... | XM_006511722.2 | 87.8% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtttgggtgt gtgactctgg gcgacaagaa gaactataac cagccatcgg 121 aggtgactga cagatatgat ttgggacagg tcatcaagac tgaggagttt tgtgaaatct 181 tccgggccaa ggacaagacg acaggcaagc tgcacacctg caagaagttc cagaagcggg 241 acggccgcaa ggtgcggaaa gctgccaaga acgagatagg catcctcaag atggtgaagc 301 atcccaacat cctacagctg gtggatgtgt ttgtgacccg caaggagtac tttatcttcc 361 tggagctggc cacggggagg gaggtgtttg actggatcct ggaccagggc tactactcgg 421 agcgagacac aagcaacgtg gtacggcaag tcctggaggc cgtggcctat ttgcactcac 481 tcaagatcgt gcacaggaat ctcaagctgg agaacctggt ttactacaac cggctgaaga 541 actcgaagat tgtcatcagt gacttccatc tggctaagct agaaaatggc ctcatcaagg 601 agccctgtgg gacccccgag tatctggccc cagaggtggt aggccggcag cggtatggac 661 gccctgtgga ctgctgggcc attggagtca tcatgtacat cctgctttca ggcaatccac 721 ctttctatga ggaggtggaa gaagatgatt atgagaacca tgataagaat ctcttccgca 781 agatcctggc tggtgactat gagtttgact ctccatattg ggatgatatt tcgcaggcag 841 ccaaagacct ggtcacaagg ctgatggagg tggagcaaga ccagcggatc actgcagaag 901 aggccatctc ccatgagtgg atttctggca atgctgcttc tgataagaac atcaaggatg 961 gtgtctgtgc ccagattgaa aagaactttg ccagggccaa gtggaagaag gctgtccgag 1021 tgaccaccct catgaaacgg ctccgggcac cagagcagtc cagcacggct gcagcccagt 1081 cggcctcagc cacagacact gccacccccg gggctgcagg tggggccaca gctgcagctg 1141 cgagtggagc taccTCAGCC CCTGAGGGTG ATGCTGCTCG TGCTGCAAAG AGTGATAATG 1201 TGGCCCCCGC AGACCGTAGT GCCACCCCAG CCACAGATGG AAGTGCCACC CCAGCCACTG 1261 ATGGCAGTGT CACCCCAGCC ACCGATGGAA GCATCACTCC AGCCACTGAT GGGAGTGTCA 1321 CCCCAGCCAC TGACAGGAGC GCTACTCCAG CCACTGATGG GAGAGCCACA CCAGCCACAG 1381 AAGAGAGCAC TGTGCCCACC ACCCAAAGCA GTGCCATGCT GGCCACCAAG GCAGCTGCCA 1441 CCCCTGAGCC GGCTATGGCC CAGCCGGACA GCACAGCCCC AGAGGGCGCC ACAGGCCAGG 1501 CTCCACCCTC TAGTAAAGGG GAAGAGGCTG CTGGTTGTGC CCAGGAGTCT CAAAGGGAGG 1561 AGGCCAGCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCGAG TATTGTATCT AGCTCCCGAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt