Construct: ORF TRCN0000470731
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010449.1_s317c1
- Derived from:
- ccsbBroadEn_12854
- DNA Barcode:
- ACAATCTGAACATGTGTTTCTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF577 (84765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470731
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370447.1 | 98.2% | 97.9% | (many diffs) |
| 2 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370448.1 | 98.2% | 97.9% | (many diffs) |
| 3 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370449.1 | 98.2% | 97.9% | (many diffs) |
| 4 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_032679.2 | 98.2% | 97.9% | (many diffs) |
| 5 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001135590.1 | 86.2% | 85.9% | (many diffs) |
| 6 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370450.1 | 86.2% | 85.9% | (many diffs) |
| 7 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370456.1 | 20.1% | 17.9% | (many diffs) |
| 8 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370457.1 | 20.1% | 17.9% | (many diffs) |
| 9 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370452.1 | 19.7% | 18.2% | (many diffs) |
| 10 | human | 84765 | ZNF577 | zinc finger protein 577 | NM_001370455.1 | 19.7% | 18.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgaggaga gagcaaggca gttcttcagg ggaggggtca ttgtcattcg 121 aagatgtggc tgtgggcttc accagggagg agtggcagtt tttggaccag tctcagaagg 181 tcttgtacaa ggaagtaatg ttggagaact acatcaacct agtatcaata gggtatcgag 241 gcaccaagcc agattcgctc ttcaagttgg agcaaggaga acccccaggg atagtagaag 301 gagcagccca cagtcaaatc tgtccaggtt ttgttatcca gagtagaaga tatgcaggaa 361 aagattctga tgcatttggt ggatatggga gatcatgcct ccatatcaag tgtgacaaaa 421 ctcttactgg agttaaatac catagatgtg ttaaacccag cagccctaaa tcacagctca 481 atgacctaca aaaaatttgt gcaggaggga aaccacatga atgcagtgtg tgcgggagag 541 ccttctccag gaaagcacag cttattcaac atcagagaac tgaaagagga gagaaacccc 601 atggatgtgg tgaatgtggg aaaacattca tgaggaagat tcagctcact gagcatcaga 661 gaactcacac aggagagaag ccccatgaat gtagtgaatg tggaaaagcc ttctccagaa 721 agtcacagct catggtccat cagagaaccc atacaggaga gaaaccctac agatgcagcg 781 aatgcggaaa agccttcagc cggaagtgcc ggctcaatag acatcagcga tcacatactg 841 gagagaaact ctatgggtgc agtgtgtgtg ggaaagcctt ttctcagaag gcatacctca 901 ctgcacacca gagacttcac acaggagata agccttataa atgcagtgat tgtggaagaa 961 ccttctattt taagtcagac ctgaccagac atcagaggat tcatacggga gagaaaccTT 1021 ATGAATGTAG TGAGTGTGAA AAAGCCTTTA GAAGCAAGTC GAAGCTCATT CAGCATCAGC 1081 GTACTCACAC TGGAGAGAGA CCATATTCAT GCAGAGAATG TGGCAAAGCC TTTGCCCACA 1141 TGTCAGTCCT CATTAAACAT GAGAAAACTC ACATAAGAGA GACAGCCATA AATTCACTGA 1201 CGGTGGAGAA ACCTTCCTCA AGGAGTCATA CCTCCTTATA CATGAGCGAA CTCATACAAG 1261 AGCAAAAGAC TGTGAACACA GTACCTATAG AAATGCCTTC CTCAGGAACC CCGCCATTGT 1321 TAAACAAGAG TGAGCGCCTA GTGGGCAGAA ATGTAGTGAT TGTGGAACAA CCTTTTCCAA 1381 GAAATCAAGC CTTTGTAGTT AATCAGGAAT TTGAACAGAG AATAAGCCTC ACAAATGAAG 1441 TGAATGTGGC CCCATCAGTA ATAAATTATA TCTTGTATCT TACAGATATT GTATCAGAAT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGAACAAT CTGAACATGT GTTTCTGTCA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt