Construct: ORF TRCN0000470736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012234.1_s317c1
- Derived from:
- ccsbBroadEn_01223
- DNA Barcode:
- AATTCCACACCCAGCAGGCGCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLS3 (5358)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5358 | PLS3 | plastin 3 | NM_001136025.5 | 100% | 100% | |
| 2 | human | 5358 | PLS3 | plastin 3 | NM_005032.7 | 100% | 100% | |
| 3 | human | 5358 | PLS3 | plastin 3 | NM_001172335.3 | 95.7% | 95.7% | 156_157ins81 |
| 4 | human | 5358 | PLS3 | plastin 3 | NM_001282337.2 | 95% | 94.8% | (many diffs) |
| 5 | human | 5358 | PLS3 | plastin 3 | NM_001282338.2 | 92.8% | 92.8% | 0_1ins135 |
| 6 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | NM_001166453.1 | 90.7% | 99% | (many diffs) |
| 7 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | NM_001166454.2 | 90.7% | 99% | (many diffs) |
| 8 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | NM_145629.2 | 90.7% | 99% | (many diffs) |
| 9 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | NM_001346519.1 | 89.5% | 97.6% | (many diffs) |
| 10 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | NM_001346520.1 | 89.5% | 97.6% | (many diffs) |
| 11 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | XM_006527733.3 | 89.5% | 97.6% | (many diffs) |
| 12 | mouse | 102866 | Pls3 | plastin 3 (T-isoform) | XM_006527731.3 | 88.9% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1956
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgagatggct accactcaga tttccaaaga tgagcttgat gaactcaaag 121 aggcctttgc aaaagttgat ctcaacagca acggattcat ttgtgactat gaacttcatg 181 agctcttcaa ggaagctaat atgccattac caggatataa agtgagagaa attattcaga 241 aactcatgct ggatggtgac aggaataaag atgggaaaat aagttttgac gaatttgttt 301 atatttttca agaggtaaaa agtagtgata ttgccaagac cttccgcaaa gcaatcaaca 361 ggaaagaagg tatttgtgct ctgggtggaa cttcagagtt gtccagcgaa ggaacacagc 421 attcttactc agaggaagaa aaatatgctt ttgttaactg gataaacaaa gctttggaaa 481 atgatcctga ttgtagacat gttataccaa tgaaccctaa caccgatgac ctgttcaaag 541 ctgttggtga tggaattgtg ctttgtaaaa tgattaacct ttcagttcct gataccattg 601 atgaaagagc aatcaacaag aagaaactta cacccttcat cattcaggaa aacttgaact 661 tggcactgaa ctctgcttct gccattgggt gtcatgttgt gaacattggt gcagaagatt 721 tgagggctgg gaaacctcat ctggttttgg gactgctttg gcagatcatt aagatcggtt 781 tgttcgctga cattgaatta agcaggaatg aagccttggc tgctttactc cgagatggtg 841 agactttgga ggaacttatg aaattgtctc cagaagagct tctgcttaga tgggcaaact 901 ttcatttgga aaactcgggc tggcaaaaaa ttaacaactt tagtgctgac atcaaggatt 961 ccaaagccta tttccatctt ctcaatcaaa tcgcaccaaa aggacaaaag gaaggtgaac 1021 cacggataga tattaacatg tcaggtttca atgaaacaga tgatttgaag agagctgaga 1081 gtatgcttca acaagcagat aaattaggtt gcagacagtt tgttacccct gctgatgttg 1141 tcagtggaaa ccccaaactc aacttagctt tcgtggctaa cctgtttaat aaatacccag 1201 cactaactaa gccagagaac caggatattg actggactct attagaagga gaaactcgtg 1261 aagaaagaac cttccgtaac tggatgaact ctcttggtgt caatcctcac gtaaaccatc 1321 tctatgctga cctgcaagat gccctggtaa tcttacagtt atatgaacga attaaagttc 1381 ctgttgactg gagtaaggtt aataaacctc catacccgaa actgggagcc aacatgaaaa 1441 agctagaaaa ctgcaactat gctgttgaat tagggaagca tcctgctaaa ttctccctgg 1501 ttggcattgg agggcaagac cTGAATGATG GGAACCAAAC CCTGACTTTA GCTTTAGTCT 1561 GGCAGCTGAT GAGAAGATAT ACCCTCAATG TCCTGGAAGA TCTTGGAGAT GGTCAGAAAG 1621 CCAATGACGA CATCATTGTG AACTGGGTGA ACAGAACGTT GAGTGAAGCT GGAAAATCAA 1681 CTTCCATTCA GAGTTTTAAG GACAAGACGA TCAGCTCCAG TTTGGCAGTT GTGGATTTAA 1741 TTGATGCCAT CCAGCCAGGC TGTATAAACT ATGACCTTGT GAAGAGTGGC AATCTAACAG 1801 AAGATGACAA GCACAATAAT GCCAAGTATG CAGTGTCAAT GGCTAGAAGA ATCGGAGCCA 1861 GAGTGTATGC TCTCCCTGAA GACCTTGTGG AAGTAAAGCC CAAGATGGTC ATGACTGTGT 1921 TTGCATGTTT GATGGGCAGG GGAATGAAGA GAGTGTACCC AACTTTCTTG TACAAAGTGG 1981 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2041 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2101 AAATTCCACA CCCAGCAGGC GCCACACGCG TTAAGTCgac aatcaacctc tggattacaa 2161 aatttgtgaa agatt