Construct: ORF TRCN0000470738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007056.1_s317c1
- Derived from:
- ccsbBroadEn_00462
- DNA Barcode:
- GTGATTTATATAACACTGGTAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DR1 (1810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1810 | DR1 | down-regulator of transcrip... | NM_001938.3 | 100% | 100% | |
| 2 | mouse | 13486 | Dr1 | down-regulator of transcrip... | NM_026106.4 | 93.1% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcctcgtct ggcaacgatg atgatctcac taTCCCCAGA GCTGCTATCA 121 ATAAAATGAT CAAAGAGACT CTTCCTAATG TCCGGGTGGC CAACGATGCT CGAGAGCTGG 181 TGGTGAACTG CTGCACTGAA TTCATTCACC TTATATCTTC TGAAGCCAAT GAGATTTGTA 241 ACAAATCGGA AAAGAAGACC ATCTCACCAG AGCATGTCAT ACAAGCACTA GAAAGTTTGG 301 GATTTGGCTC TTACATCAGT GAAGTAAAAG AAGTCTTGCA AGAGTGTAAA ACAGTAGCAT 361 TAAAAAGAAG AAAGGCCAGT TCTCGTTTGG AAAACCTTGG CATTCCTGAA GAAGAGTTAT 421 TGAGACAGCA ACAAGAATTA TTTGCAAAAG CTAGACAGCA ACAAGCAGAA TTGGCCCAAC 481 AGGAATGGCT TCAAATGCAG CAAGCTGCCC AACAAGCCCA GCTTGCTGCT GCCTCAGCCA 541 GTGCATCTAA TCAGGCGGGA TCTTCTCAGG ATGAAGAAGA TGATGATGAT ATCTGCCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAG TGATTTATAT AACACTGGTA AACACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att