Construct: ORF TRCN0000470761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010409.1_s317c1
- Derived from:
- ccsbBroadEn_11839
- DNA Barcode:
- CTATACACCATTGACTGTCGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPHP3 (27031)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27031 | NPHP3 | nephrocystin 3 | NM_153240.5 | 10.8% | 10.1% | (many diffs) |
| 2 | human | 100532724 | NPHP3-ACAD11 | NPHP3-ACAD11 readthrough (N... | NR_037804.1 | 5.5% | (many diffs) | |
| 3 | mouse | 74025 | Nphp3 | nephronophthisis 3 (adolesc... | NM_172460.1 | 27.2% | 26.6% | (many diffs) |
| 4 | mouse | 74025 | Nphp3 | nephronophthisis 3 (adolesc... | NM_028721.3 | 9.6% | 9.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaccgcctcg tcgctcgtga gccccgcggg cggggaagtg atcgaggaca 121 cgtacggggc gggcggcggc gaggcctgcg agatcccggt ggaggtgaag cccaaggccc 181 gccTGCTGCG CAACTCGTTC CGCCGAGGCG CGGGGGCGGC AGCAGGGGCC GGGCCCGGGT 241 CGCTGCCCCG CGGGGTGGGC GCGGGCGGGC TGCTGGGGGC CAGCTTCAAG TCCACTGGCT 301 CGTCGGTGCC AGAGCTGGAG TACGCGGCGG CCGAGTACGA GCGGCTCAGG AAGGAGTACG 361 AGATCTTTCG CGTCAGCAAG AACCAGGAGT TGTTGTCCAT GGGCCGCCGC GAGGCCAAGC 421 TGGACACGGA GAACAAGCGG CTGCGGGCCG AACTGCAGGG TCTCGCTGCG GTGGCACGAT 481 CGCGGCTCAC TGCAACCTGG AACTCCTGGG CTCAATGCCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 ACTATACACC ATTGACTGTC GGCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt