Transcript: Human NM_153240.5

Homo sapiens nephrocystin 3 (NPHP3), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
NPHP3 (27031)
Length:
5348
CDS:
55..4047

Additional Resources:

NCBI RefSeq record:
NM_153240.5
NBCI Gene record:
NPHP3 (27031)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_153240.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000118612 CGTGTGATTTAACTGCTATTT pLKO.1 4154 3UTR 100% 13.200 18.480 N NPHP3 n/a
2 TRCN0000366390 GTGCGAGACAATGGGATATTT pLKO_005 1038 CDS 100% 15.000 7.500 Y Nphp3 n/a
3 TRCN0000118614 CCTCCCTTATTCACAGTTTAT pLKO.1 2453 CDS 100% 13.200 6.600 Y NPHP3 n/a
4 TRCN0000118615 GCTTAGCTGATCTTTATGAAA pLKO.1 2876 CDS 100% 5.625 2.813 Y NPHP3 n/a
5 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 4487 3UTR 100% 4.950 2.475 Y ORAI2 n/a
6 TRCN0000118613 GCTGTTGAAATTCGACAGAAA pLKO.1 3661 CDS 100% 4.950 2.475 Y NPHP3 n/a
7 TRCN0000118616 GCCTTGGTGAACTTAGCTGTT pLKO.1 3715 CDS 100% 4.050 2.025 Y NPHP3 n/a
8 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 4484 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153240.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11839 pDONR223 100% 10.8% 10.1% None (many diffs) n/a
2 ccsbBroad304_11839 pLX_304 0% 10.8% 10.1% V5 (many diffs) n/a
3 TRCN0000470761 CTATACACCATTGACTGTCGGCCA pLX_317 72.9% 10.8% 10.1% V5 (many diffs) n/a
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