Construct: ORF TRCN0000470763

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009957.1_s317c1
Derived from:
ccsbBroadEn_11637
DNA Barcode:
CGCATTTGAGGATCCGCAGCACAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC31A (22872)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470763
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001300744.2 47.2% 47.1% (many diffs)
2 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001077206.3 45.7% 45.4% (many diffs)
3 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001300745.2 43.2% 43.1% (many diffs)
4 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_016211.4 42.7% 42.6% (many diffs)
5 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001077208.3 42% 41.7% (many diffs)
6 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001318119.1 42% 41.7% (many diffs)
7 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001318120.1 41.5% 41.2% (many diffs)
8 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001077207.4 41.4% 41.2% (many diffs)
9 human 22872 SEC31A SEC31 homolog A, COPII coat... NM_001191049.2 40.9% 40% (many diffs)
10 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_006535207.3 48.8% 52.2% (many diffs)
11 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249563.2 42% 45.1% (many diffs)
12 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249562.2 40.6% 43.3% (many diffs)
13 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249561.2 39.4% 42.2% (many diffs)
14 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249560.2 38.5% 41.4% (many diffs)
15 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249559.2 38% 40.8% (many diffs)
16 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249558.2 37.6% 40.4% (many diffs)
17 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249557.2 37.3% 39.9% (many diffs)
18 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_017321089.1 37.3% 39.9% (many diffs)
19 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249556.2 36.8% 39.4% (many diffs)
20 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_017321088.1 36.8% 39.4% (many diffs)
21 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249555.2 36.6% 39.4% (many diffs)
22 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... NM_026969.1 36.4% 38.9% (many diffs)
23 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249554.2 36.3% 38.8% (many diffs)
24 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249553.2 36.3% 38.8% (many diffs)
25 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249552.2 36% 38.4% (many diffs)
26 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_011249551.2 35.9% 38.4% (many diffs)
27 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_006535192.2 35.5% 38% (many diffs)
28 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_006535193.1 35.5% 38% (many diffs)
29 mouse 69162 Sec31a Sec31 homolog A (S. cerevis... XM_006535194.2 35.5% 38% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1593
ORF length:
1527
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gttaaaggaa gtagatcgta cagccatgca ggcatggagc cctgcccaga
121atcaccccat ttacctagca acaggaacat ctgctcagca attggatgca acatttagta
181cgaatgcttc ccttgagata tttgaattag acctctctga tccatccttg gatatgaaat
241cttgtgccac attctcctct tctcacaggt accacaagtt gatttggggg ccttataaaa
301tggattccaa aggagatgtc tctggagttc tgattgcagg tggtgaaaat ggaaatatta
361ttctctatga tccttctaaa attatagctg gagacaagga agttgtgatt gcccagaatg
421acaagcatac tggcccagtg agagccttgg atgtgaacat tttccagact aatctggtag
481cttctggtgc taatgaatct gaaatctaca tatgggatct aaataatttt gcaaccccaa
541tgacaccagg agccaaaaca cagccgccag aagatatcag ctgcattgca tggaacagac
601aagttcagca tattttagca tcagccagtc ccagtggccg ggccactgta tgggatctta
661gaaaaaatga gccaatcatc aaagtcagtg accatagtaa cagaatgcat tgttctgggt
721tggcatggca tcctgatgtt gctactcaga tggtccttgc ctccgaggat gaccggttac
781cagtgatcca gatgtgggat cttcgatttg cttcctctcc acttcgtgtc ctggaaaacc
841atgccagggg gattttggca attgcttgga gcatggcaga tcctgaattg ttactgagct
901gtggaaaaga tgctaagatt ctctgctcca atccaaacac aggagaggtg ttatatgaac
961ttcccaccaa cacacagtgg tgcttcgata ttcagtggtg tccccgaaat cctgctgtct
1021tatcagctgc ttcgtttgat gggcgtatca gtgtttattc tatcatggga ggaagcacag
1081atggtttaag acagaaacaa gttgacaagc tttcatcatc ttttgggaat cttgatccct
1141ttggcacagg acagcccctt cctccgttac aaatTCCACA GCAGACTGCT CAGCATAGTA
1201TAGTGCTGCC TCTGAAGAAG CCGCCCAAGT GGATTCGAAG GCCTGTTGGT GCTTCTTTTT
1261CATTTGGAGG CAAACTGGTT ACGTTTGAGA ATGTCAGAAT GCCTTCTCAT CAGGGAGCTG
1321AGCAGCAGCA GCAGCAGCAC CATGTGTTCA TTAGTCAGGT TGTAACAGAA AAGGAGTTCC
1381TCAGCCGATC AGACCAACTT CAGCAGGCTG TGCAGTCACA AGGATTTATC AATTATTGCC
1441AAAAAAAAAT TGATGCTTCT CAGACTGAAT TTGAGAAAAA TGTGTGGTCC TTTTTGAAGG
1501TAAACTTTGA GGATGATTCT CGTGGAAAAT ACCTTGAACT TCTAGGATAC AGAAAAGAAG
1561ATCTAGGAAA GAAGGTAAAC TTTTGGGAAA GTTACCCAAC TTTCTTGTAC AAAGTGGTTG
1621ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1681GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG
1741CATTTGAGGA TCCGCAGCAC AGACGCGTTA AGTCgacaat caacctctgg attacaaaat
1801ttgtgaaaga tt

Download FASTA (ORF) (Full)