Construct: ORF TRCN0000470782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015965.1_s317c1
- Derived from:
- ccsbBroadEn_07550
- DNA Barcode:
- ACATAGAGCGTCCCGCTCTGTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN2 (10100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10100 | TSPAN2 | tetraspanin 2 | NM_005725.6 | 99.8% | 100% | 228G>A |
| 2 | human | 10100 | TSPAN2 | tetraspanin 2 | NM_001308315.1 | 88.5% | 88.6% | 228G>A;269_270ins75 |
| 3 | human | 10100 | TSPAN2 | tetraspanin 2 | NM_001308316.1 | 87.1% | 87.3% | 228G>A;515_516ins84 |
| 4 | human | 10100 | TSPAN2 | tetraspanin 2 | XM_016999996.1 | 75.8% | 76% | 228G>A;269_270ins75;440_441ins84 |
| 5 | mouse | 70747 | Tspan2 | tetraspanin 2 | NM_027533.3 | 88.6% | 93.6% | (many diffs) |
| 6 | mouse | 70747 | Tspan2 | tetraspanin 2 | NM_001243132.1 | 83.2% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcgcttccgc gggggcctgc ggtgcatcaa gtacctgctg cttggcttca 121 acctgctctt ctggctggct ggatcggccg tcattgcttt tggactatgg tttcggttcg 181 gaggtgccat aaaggagtta tcatcagagg acaagtcccc agagtatttc tatgtggggc 241 tgtatgttct ggttggagcc ggggcccTGA TGATGGCCGT GGGGTTCTTC GGATGCTGCG 301 GAGCCATGCG GGAGTCGCAA TGTGTGCTTG GATCATTTTT TACCTGCCTC CTGGTGATAT 361 TTGCTGCTGA AGTAACCACT GGAGTATTTG CTTTTATAGG CAAGGGGGTA GCTATCCGAC 421 ATGTTCAGAC CATGTATGAA GAGGCTTACA ATGATTACCT TAAAGACAGG GGAAAAGGCA 481 ATGGGACACT CATCACCTTC CACTCAACAT TTCAGTGCTG TGGAAAAGAA AGCTCCGAAC 541 AGGTCCAACC TACATGCCCA AAGGAGCTTC TAGGACACAA GAATTGCATC GATGAAATTG 601 AGACCATAAT CAGTGTTAAG CTCCAGCTCA TTGGAATTGT CGGTATTGGA ATTGCAGGTC 661 TGACGATCTT TGGCATGATA TTCAGCATGG TCCTCTGCTG TGCGATACGA AACTCACGAG 721 ATGTGATATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACATAG AGCGTCCCGC TCTGTTAAAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt