Construct: ORF TRCN0000470789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009920.1_s317c1
- Derived from:
- ccsbBroadEn_15556
- DNA Barcode:
- AGCCACCCTCGGCCAACCTCGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCD3 (5825)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470789
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5825 | ABCD3 | ATP binding cassette subfam... | NM_001122674.1 | 100% | 100% | |
| 2 | human | 5825 | ABCD3 | ATP binding cassette subfam... | NM_002858.4 | 35.3% | 34.5% | (many diffs) |
| 3 | human | 5825 | ABCD3 | ATP binding cassette subfam... | XM_006710802.2 | 34% | 33.3% | (many diffs) |
| 4 | mouse | 19299 | Abcd3 | ATP-binding cassette, sub-f... | NM_008991.2 | 32.6% | 33.5% | (many diffs) |
| 5 | mouse | 19299 | Abcd3 | ATP-binding cassette, sub-f... | XM_006501152.2 | 22.6% | 22.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccttcagc aagtacttga cggcgcgaaa ctcctcgctg gctggtgccg 121 cgttcctgct gctctgcctg ctccacaagc ggcgccgcgc cctcggcctg cacggtaaga 181 aaagtggaaa accaccatta cagaacaatg agaaagaggg gaaaaaggag cgagctgtgg 241 tggacaaggt gtttttctca aggctcatac agattctgaa aatcatggtc cctagaacat 301 tttGTAAAGA GACAGGTTAC TTGGTACTTA TTGCTGTTAT GCTGGTGTCT CGAACATATT 361 GTGATGTTTG GATGATTCAA AATGGGACAC TAATTGAAAG TGGTATCATT GGTCGTAGCA 421 GGAAAGATTT CAAGAGATAC TTACTCAACT TCATCGCTGC CATGCCTCTT ATCTCTCTGG 481 TTAATAACTT CTTGAAGTAT GGGTTAAATG AGCTTAAACT GTGCTTCCGA GTAAGGCTCA 541 CTAAATACCT CTATGAGGAG TATCTTCAAG CTTTCACATA TTATAAAATG GGGAATCTGG 601 ACAACAGAAT AGCTAATCCA GACCAGCTGC TTACACAAGA TGTAGAAAAA TTTTGTAACA 661 GTGTAGTCGA TCTGTATTCA AATCTTAGTA AGCCATTTTT AGACATAGTT TTGTATATCT 721 TTAAGTTAAC GAGTGCAATT GGAGCTCAGG TACTTGGAAA AATTTTGTGG CATTACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAA GCCACCCTCG GCCAACCTCG CGAACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att