Construct: ORF TRCN0000470815
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005501.1_s317c1
- Derived from:
- ccsbBroadEn_02814
- DNA Barcode:
- CATGGCTCTAGCCCCATGACTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KPNA6 (23633)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470815
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23633 | KPNA6 | karyopherin subunit alpha 6 | NM_012316.5 | 100% | 100% | |
| 2 | human | 23633 | KPNA6 | karyopherin subunit alpha 6 | XM_024446165.1 | 99.4% | 99.4% | 0_1insATGGAGACC |
| 3 | human | 23633 | KPNA6 | karyopherin subunit alpha 6 | XM_024446166.1 | 99.4% | 99.4% | 0_1insATGGAGACC |
| 4 | human | 23633 | KPNA6 | karyopherin subunit alpha 6 | XM_024446167.1 | 99.4% | 99.4% | 0_1insATGGAGACC |
| 5 | human | 23633 | KPNA6 | karyopherin subunit alpha 6 | XM_005270711.3 | 94.6% | 94.5% | 1_85del;89_93delTGTCA |
| 6 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | XM_024446426.1 | 46.7% | 54.3% | (many diffs) |
| 7 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | XM_024446427.1 | 46.7% | 54.3% | (many diffs) |
| 8 | mouse | 16650 | Kpna6 | karyopherin (importin) alpha 6 | NM_008468.4 | 91.7% | 99.2% | (many diffs) |
| 9 | mouse | 16650 | Kpna6 | karyopherin (importin) alpha 6 | XM_006502814.3 | 90.9% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1674
- ORF length:
- 1608
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaccatggcg agcccaggga aagacaatta tcgaatgaag agctataaga 121 acaatgctct aaaccctgaa gaaatgagac gaagaagaga ggaagagggc attcagctcc 181 ggaagcagaa gcgagagcaa caacttttta aacggagaaa tgtggagctg attaatgaag 241 aagctgccat gttcgatagt cttctcatgg actcttatgt gagctctacc actggggaga 301 gtgtgatcac aagagagatg gtggagatgc tcttttctga tgattctgac ctgcagttag 361 caaccacaca gaaattccgg aaactgctct ccaaagagcc tagtcctcca atagatgaag 421 ttatcaacac tccaagagtg gtggatcggt tcgtggagtt tctgaagagg aatgagaatt 481 gtacattaca gtttgaagct gcctgggctc taacgaatat tgcctctgga acctctcagc 541 agaccaaaat tgtcattgaa gcaggggctg tccccatttt tatagagctg cttaattcag 601 actttgagga tgttcaggaa caggcagtct gggcactggg aaacatagct ggagatagct 661 ctgtttgccg agattacgtc ttgaactgtt ccatccttaa tcctttgtta acactcctta 721 ccaagtccac acgactgacg atgacacgga atgcagtctg ggccctgtca aatctctgcc 781 gagggaaaaa cccaccccca gagtttgcaa aggtctctcc ttgtttgcct gtactgtctc 841 gcctactctt cagcagcgac tcggacttgc tggcagatgc ttgctgggcc ctttcttatc 901 tgtctgatgg ccccaatgag aagatccagg cagtcataga ctccggagtc tgccggagat 961 tggtagagct gctgatgcac aatgattaca aagtggcttc tcctgccctg agagccgtgg 1021 gtaacatcgt cactggggat gacatccaga cccaggtcat tcttaactgt tcagccctac 1081 cttgccttct ccacttgttg agcagtccca aggagtcaat ccggaaggaa gcttgctgga 1141 ctatttcaaa tattactgct ggcaacaggg ctcaaataca ggctgttata gatgcaaata 1201 tcttccctgt gttgatcgaa atccttcaga aagcagagtt tcGTACAAGG AAAGAGGCAG 1261 CCTGGGCCAT CACCAATGCC ACATCAGGAG GAACCCCTGA GCAGATCAGG TACCTGGTCT 1321 CACTGGGCTG CATCAAACCC CTATGTGACT TGCTGACTGT AATGGATTCG AAGATTGTGC 1381 AAGTGGCCCT CAATGGACTG GAGAACATCC TGCGGCTTGG AGAGCAAGAG GGCAAGCGCA 1441 GTGGCTCAGG GGTCAATCCT TATTGTGGCC TCATAGAGGA AGCCTATGGC TTGGATAAAA 1501 TTGAGTTTCT CCAGAGCCAC GAGAACCAGG AGATCTACCA GAAGGCCTTC GACCTCATTG 1561 AGCACTACTT TGGTGTAGAA GACGATGATA GCAGCCTGGC TCCCCAAGTC GATGAAACGC 1621 AACAGCAGTT CATCTTCCAG CAGCCTGAGG CCCCCATGGA GGGCTTCCAG CTATACCCAA 1681 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1741 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1801 TATCTTGTGG AAAGGACGAC ATGGCTCTAG CCCCATGACT GTCACGCGTT AAGTCgacaa 1861 tcaacctctg gattacaaaa tttgtgaaag att