Transcript: Mouse XM_006502814.3

PREDICTED: Mus musculus karyopherin (importin) alpha 6 (Kpna6), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kpna6 (16650)
Length:
5715
CDS:
83..1708

Additional Resources:

NCBI RefSeq record:
XM_006502814.3
NBCI Gene record:
Kpna6 (16650)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006502814.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000313193 TGGAGGGCTTCCAGCTATAAT pLKO_005 1689 CDS 100% 15.000 21.000 N Kpna6 n/a
2 TRCN0000313124 CTTGAGAGCTGTGGGTAATAT pLKO_005 1039 CDS 100% 15.000 10.500 N Kpna6 n/a
3 TRCN0000313126 TTCTTGCTCTGACCCATATTA pLKO_005 2203 3UTR 100% 15.000 10.500 N Kpna6 n/a
4 TRCN0000313195 AGTTAGCAACCACGCAGAAAT pLKO_005 387 CDS 100% 13.200 9.240 N Kpna6 n/a
5 TRCN0000093284 CCTCCAATAGATGAAGTTATT pLKO.1 437 CDS 100% 13.200 9.240 N Kpna6 n/a
6 TRCN0000093286 CCCAGGTCATTCTTAATTGTT pLKO.1 1083 CDS 100% 5.625 3.938 N Kpna6 n/a
7 TRCN0000312084 CCCAGGTCATTCTTAATTGTT pLKO_005 1083 CDS 100% 5.625 3.938 N Kpna6 n/a
8 TRCN0000093288 CCACCTACTGAGCAGCTCTAA pLKO.1 1123 CDS 100% 4.950 3.465 N Kpna6 n/a
9 TRCN0000093285 GCTCAAATACAGGCTGTCATA pLKO.1 1202 CDS 100% 4.950 3.465 N Kpna6 n/a
10 TRCN0000093287 CCAGGAGTAGTTGATCGGTTT pLKO.1 464 CDS 100% 4.050 2.835 N Kpna6 n/a
11 TRCN0000293167 GAGGGCTTCCAGCTATAATAT pLKO_005 1691 CDS 100% 15.000 10.500 N KPNA6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006502814.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02814 pDONR223 100% 90.9% 98.1% None (many diffs) n/a
2 ccsbBroad304_02814 pLX_304 0% 90.9% 98.1% V5 (many diffs) n/a
3 TRCN0000470815 CATGGCTCTAGCCCCATGACTGTC pLX_317 27.1% 90.9% 98.1% V5 (many diffs) n/a
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