Construct: ORF TRCN0000470820
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011917.1_s317c1
- Derived from:
- ccsbBroadEn_10190
- DNA Barcode:
- CGGCCAATTTTGTTAATTTGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCF1 (653361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470820
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653361 | NCF1 | neutrophil cytosolic factor 1 | NM_000265.6 | 100% | 100% | |
| 2 | human | 654817 | NCF1C | neutrophil cytosolic factor... | NR_003187.3 | 79.4% | (many diffs) | |
| 3 | human | 654816 | NCF1B | neutrophil cytosolic factor... | NR_003186.1 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacaccttc atccgtcaca tcgccctgct gggctttgag aagcgcttcg 121 tacccagcca gcactatgtg tacatgttcc tggtgaaatg gcaggacctg tcggagaagg 181 tggtctaccg gcgcttcacc gagatctacg agttccataa aaccttaaaa gaaatgttcc 241 ctattgaggc aggggcgatc aatccagaga acaggatcat cccccacctc ccagctccca 301 agtggtttga cgggcagcgg gccgccgaga accgccaggg cacacttacc gagtactgca 361 gcacgctcat gagcctgccc accaagatct cccgctgtcc ccacctcctc gacttcttca 421 aggtgcgccc tgatgacctc aagctcccca cggacaacca gacaaaaaag ccagagacat 481 acttgatgcc caaagatggc aagagtaccg cgacagacat caccggcccc atcatcctgc 541 agacgtaccg cgccattgcc aactacgaga agacctcggg ctccgagatg gctctgtcca 601 cgggggacgt ggtggaggtc gtagagaaga gcgagagcgg ttggtggttc tgtcagatga 661 aagcaaagcg aggctggatc ccagcgtcct tcctcgagcc cctggacagt cctgacgaga 721 cggaagaccc tgagcccaac tatgcaggtg agccatacgt cgccatcaag gcctacactg 781 ctgtggaggg ggacgaggtg tccctgctcg agggtgaagc tgttgaggtc attcacaagc 841 tcctggacgg ctggtgggtc atcaggaaag acgacgtcac aggctacttc ccgtccatgt 901 acctgcaaaa gtcagggcaa gacgtgtccc aggcccaacg ccagatcaag cggggggcgc 961 cgccccgcag gtcgtccatc cgcaacgcgc acagcatcca ccagcggtcg cggaagcgcc 1021 tcagccagga cgcctatcgc cgcaacagcg tccgttttct gcagcagcga cgccgccagg 1081 cgcggccggg accgcagagc cccgggagcc cgctcgagga ggagcggcag acgcagcgct 1141 ctaaaccgca gccggcggtg cccccgcggc cgagcgccga cctcatcctg aaccgctgca 1201 gcgagagcac caagcggaag ctggcgTCTG CCGTCTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 ACGGCCAATT TTGTTAATTT GTTACACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt