Transcript: Human NR_003186.1

Homo sapiens neutrophil cytosolic factor 1B pseudogene (NCF1B), non-coding RNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
NCF1B (654816)
Length:
1552
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_003186.1
NBCI Gene record:
NCF1B (654816)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_003186.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256332 TGTACATGTTCCTGGTGAAAT pLKO_005 79 3UTR 100% 13.200 6.600 Y NCF1 n/a
2 TRCN0000256334 GGTGGTTCTGTCAGATGAAAG pLKO_005 804 3UTR 100% 10.800 5.400 Y NCF1 n/a
3 TRCN0000256331 CCATTGCCAACTACGAGAAGA pLKO_005 251 3UTR 100% 4.950 2.475 Y NCF1 n/a
4 TRCN0000038926 GTTCTGTCAGATGAAAGCAAA pLKO.1 808 3UTR 100% 4.950 2.475 Y NCF1C n/a
5 TRCN0000038924 GAGACATACTTGATGCCCAAA pLKO.1 172 3UTR 100% 4.050 2.025 Y NCF1C n/a
6 TRCN0000038925 CCGAGATCTACGAGTTCCATA pLKO.1 139 3UTR 100% 4.950 2.475 Y NCF1C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_003186.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10190 pDONR223 100% 48.2% None (many diffs) n/a
2 ccsbBroad304_10190 pLX_304 0% 48.2% V5 (many diffs) n/a
3 TRCN0000470820 CGGCCAATTTTGTTAATTTGTTAC pLX_317 1.6% 48.2% V5 (many diffs) n/a
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