Construct: ORF TRCN0000470865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015012.1_s317c1
- Derived from:
- ccsbBroadEn_02149
- DNA Barcode:
- GCTAGCCCATGTTGTCTCGCCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB33A (9363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9363 | RAB33A | RAB33A, member RAS oncogene... | NM_004794.3 | 100% | 100% | |
| 2 | human | 9363 | RAB33A | RAB33A, member RAS oncogene... | XM_017029963.2 | 64.1% | 63.7% | (many diffs) |
| 3 | mouse | 19337 | Rab33a | RAB33A, member RAS oncogene... | NM_011228.2 | 92.2% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagcccatc ctgggccatg ggagcctgca gcccgcctcg gccgctggcc 121 tggcgtccct ggagctcgac tcgtcgctgg accagtacgt gcagattcgc atcttcaaaa 181 taatcgtgat tggggactcc aacgtgggca agacctgcct gaccttccgc ttctgcgggg 241 gtaccttccc agacaagact gaagccacca tcggcgtgga cttcagggag aagaccgtgg 301 aaatcgaggg cgagaagatc aaggttcagg tgtgggacac agcaggTCAG GAACGTTTCC 361 GCAAAAGCAT GGTCGAGCAT TACTACCGCA ACGTACATGC CGTGGTCTTC GTCTATGACG 421 TCACCAAGAT GACATCTTTC ACCAACCTCA AAATGTGGAT CCAAGAATGC AATGGGCATG 481 CTGTGCCCCC ACTAGTCCCC AAAGTGCTTG TGGGCAACAA GTGTGACTTG AGGGAACAGA 541 TCCAGGTGCC CTCCAACTTA GCCCTGAAAT TTGCTGATGC CCACAACATG CTCTTGTTTG 601 AGACATCGGC CAAGGACCCC AAAGAGAGCC AGAACGTGGA GTCGATTTTC ATGTGCTTGG 661 CTTGCCGATT GAAGGCCCAG AAATCCCTGC TGTATCGTGA TGCTGAGAGG CAGCAGGGGA 721 AGGTGCAGAA ACTGGAGTTC CCACAGGAAG CTAACAGTAA AACTTCCTGT CCTTGTTGCC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAGCTAGCCC ATGTTGTCTC GCCTAGACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt