Construct: ORF TRCN0000470870

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012875.1_s317c1
Derived from:
ccsbBroadEn_09818
DNA Barcode:
ATCGAAAGGTTTTTACTTCCCAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DENND6A (201627)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470870
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 201627 DENND6A DENN domain containing 6A NM_152678.3 99.9% 100% 1029C>G
2 human 201627 DENND6A DENN domain containing 6A XM_006713019.3 99.7% 99.8% 429_430insAAG;1026C>G
3 human 201627 DENND6A DENN domain containing 6A XM_006713020.2 87% 87.3% (many diffs)
4 human 201627 DENND6A DENN domain containing 6A XM_017005864.1 86.8% 87.1% (many diffs)
5 human 201627 DENND6A DENN domain containing 6A XR_245100.1 49.6% 1_71del;1100C>G;1896_3670del
6 human 201627 DENND6A DENN domain containing 6A XR_001740057.2 46.8% (many diffs)
7 mouse 211922 Dennd6a DENN/MADD domain containing 6A NM_001134465.2 90.6% 94.9% (many diffs)
8 mouse 211922 Dennd6a DENN/MADD domain containing 6A NM_145969.4 83.5% 84.7% (many diffs)
9 mouse 211922 Dennd6a DENN/MADD domain containing 6A NM_001285466.1 56.7% 60.1% (many diffs)
10 mouse 211922 Dennd6a DENN/MADD domain containing 6A NM_001285467.1 56.7% 60.1% (many diffs)
11 mouse 211922 Dennd6a DENN/MADD domain containing 6A XM_006518778.1 56.7% 60.1% (many diffs)
12 mouse 211922 Dennd6a DENN/MADD domain containing 6A XM_006518779.1 56.7% 60.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1890
ORF length:
1824
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tttgaggggc cctgcgggct tggggcccgg ctctcgaagg ccgttggacg
121aagcggtggc aggggccgag ggccgcgagg cgccggccct tgtggcggcg ggaggcgcgc
181cagaggacga tgaagaggac gatggccgtg gccggggcct gctgcgctgg gacagcttct
241ccgcctggct gcactgcgtg tgtgtggtgg gcttcgacct ggagctgggc caggccgtgg
301aggtaattta tcctcagcat tccaaactta ctgacagaga aaaaaccaat atttgctatt
361tgtcttttcc agattcaaat tcaggttgtc ttggagatac ccagttttgt tttagatttc
421gacagtcttc tgggaggagg gtgtcgctgc attgtctcct ggatcaattt gacaaagatt
481taccagttta cttaaagaag gatcctgctt atttttatgg atatgtgtat ttccgacaag
541ttcgagataa aactctaaaa agaggctact ttcagaagtc cttggttttg atcagcaaac
601taccttatat tcattttttt cacactgtgc tcaaacagat agcaccagag tattttgaaa
661agaatgaacc ttatttggaa gcagcttgta atgatgttga tcgatggcct gccccagtgc
721cagggaaaac attacacctg ccaatcatgg gggtggtaat gaaggtacgg attcccacat
781gtcatgacaa gcctgggaca actcaaatag tgcagttaac tcagcaggtg gacacaaata
841tatctgttat tttacctact gttcatgagg tggatatttt caggtgtttc tgcccagttt
901tccttcatag tcagatgctc tgggagctgg tgctgttggg ggagcccctt gtggttatgg
961cgccatcacc atcggaatca tcagagactg tattggcact tgttaactgt atttctccat
1021taaagtactt cagtgatttc cgaccttatt tcactattca tgatagtgaa ttcaaagaat
1081atactacccg tacgcaagct ccgccctcag ttatattagg agtaaccaac cctttttttg
1141ctaagacact ccagcactgg ccacacatta ttcgaatagg agaccttaaa cctacaggtg
1201aaattcctaa gcaggttaaa gtgaaaaaac tgaagaatct aaagactctg gattccaaac
1261ctggagttta tacttcatat aagccatatt taaatagaga tgaagagatc ataaaacaat
1321tacagaaggg tgtacaacag aaacgtcctt ctgaggctca aagtgttatt cttcgacgct
1381attttttgga actgacacaa agtttcatca ttccattaga aagatatgtg gcaagcttga
1441tgcctttgca gaaaagtatt tccccatgga agagtccacc tcaattaaga cagtttcttc
1501cagaagaatt tatgaaaaca cttgagaaaa caggaccTCA GCTAACCTCT AGAATAAAAG
1561GCGATTGGAT TGGACTTTAC CGGCATTTCC TAAAGTCTCC AAATTTTGAT GGCTGGTTTA
1621AGACCCGGAG GAAGGAAATG ACCCAAAAAT TGGAGGCACT CCATCTAGAA GCTCTTTGTG
1681AAGAGGACTT ACTTCTCTGG ATCCAGAAAC ACACAGAAGT AGAAACAGTA GACCTTGTCT
1741TGAAGCTGAA AAATAAGCTG TTGCAGGCTG ATCGAGAGCA CTTACCTGTG AAACCTGACA
1801CTATGGAAAA GTTACGGACA CACATAGATG CCATTATCTT AGCATTGCCA GAGGACTTGC
1861AAGGCATACT GCTCAAAACG GGCATGACAT GCCCAACTTT CTTGTACAAA GTGGTTGATA
1921TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1981CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCGA
2041AAGGTTTTTA CTTCCCAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2101tgaaagatt

Download FASTA (ORF) (Full)