Construct: ORF TRCN0000470870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012875.1_s317c1
- Derived from:
- ccsbBroadEn_09818
- DNA Barcode:
- ATCGAAAGGTTTTTACTTCCCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND6A (201627)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201627 | DENND6A | DENN domain containing 6A | NM_152678.3 | 99.9% | 100% | 1029C>G |
| 2 | human | 201627 | DENND6A | DENN domain containing 6A | XM_006713019.3 | 99.7% | 99.8% | 429_430insAAG;1026C>G |
| 3 | human | 201627 | DENND6A | DENN domain containing 6A | XM_006713020.2 | 87% | 87.3% | (many diffs) |
| 4 | human | 201627 | DENND6A | DENN domain containing 6A | XM_017005864.1 | 86.8% | 87.1% | (many diffs) |
| 5 | human | 201627 | DENND6A | DENN domain containing 6A | XR_245100.1 | 49.6% | 1_71del;1100C>G;1896_3670del | |
| 6 | human | 201627 | DENND6A | DENN domain containing 6A | XR_001740057.2 | 46.8% | (many diffs) | |
| 7 | mouse | 211922 | Dennd6a | DENN/MADD domain containing 6A | NM_001134465.2 | 90.6% | 94.9% | (many diffs) |
| 8 | mouse | 211922 | Dennd6a | DENN/MADD domain containing 6A | NM_145969.4 | 83.5% | 84.7% | (many diffs) |
| 9 | mouse | 211922 | Dennd6a | DENN/MADD domain containing 6A | NM_001285466.1 | 56.7% | 60.1% | (many diffs) |
| 10 | mouse | 211922 | Dennd6a | DENN/MADD domain containing 6A | NM_001285467.1 | 56.7% | 60.1% | (many diffs) |
| 11 | mouse | 211922 | Dennd6a | DENN/MADD domain containing 6A | XM_006518778.1 | 56.7% | 60.1% | (many diffs) |
| 12 | mouse | 211922 | Dennd6a | DENN/MADD domain containing 6A | XM_006518779.1 | 56.7% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1890
- ORF length:
- 1824
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttgaggggc cctgcgggct tggggcccgg ctctcgaagg ccgttggacg 121 aagcggtggc aggggccgag ggccgcgagg cgccggccct tgtggcggcg ggaggcgcgc 181 cagaggacga tgaagaggac gatggccgtg gccggggcct gctgcgctgg gacagcttct 241 ccgcctggct gcactgcgtg tgtgtggtgg gcttcgacct ggagctgggc caggccgtgg 301 aggtaattta tcctcagcat tccaaactta ctgacagaga aaaaaccaat atttgctatt 361 tgtcttttcc agattcaaat tcaggttgtc ttggagatac ccagttttgt tttagatttc 421 gacagtcttc tgggaggagg gtgtcgctgc attgtctcct ggatcaattt gacaaagatt 481 taccagttta cttaaagaag gatcctgctt atttttatgg atatgtgtat ttccgacaag 541 ttcgagataa aactctaaaa agaggctact ttcagaagtc cttggttttg atcagcaaac 601 taccttatat tcattttttt cacactgtgc tcaaacagat agcaccagag tattttgaaa 661 agaatgaacc ttatttggaa gcagcttgta atgatgttga tcgatggcct gccccagtgc 721 cagggaaaac attacacctg ccaatcatgg gggtggtaat gaaggtacgg attcccacat 781 gtcatgacaa gcctgggaca actcaaatag tgcagttaac tcagcaggtg gacacaaata 841 tatctgttat tttacctact gttcatgagg tggatatttt caggtgtttc tgcccagttt 901 tccttcatag tcagatgctc tgggagctgg tgctgttggg ggagcccctt gtggttatgg 961 cgccatcacc atcggaatca tcagagactg tattggcact tgttaactgt atttctccat 1021 taaagtactt cagtgatttc cgaccttatt tcactattca tgatagtgaa ttcaaagaat 1081 atactacccg tacgcaagct ccgccctcag ttatattagg agtaaccaac cctttttttg 1141 ctaagacact ccagcactgg ccacacatta ttcgaatagg agaccttaaa cctacaggtg 1201 aaattcctaa gcaggttaaa gtgaaaaaac tgaagaatct aaagactctg gattccaaac 1261 ctggagttta tacttcatat aagccatatt taaatagaga tgaagagatc ataaaacaat 1321 tacagaaggg tgtacaacag aaacgtcctt ctgaggctca aagtgttatt cttcgacgct 1381 attttttgga actgacacaa agtttcatca ttccattaga aagatatgtg gcaagcttga 1441 tgcctttgca gaaaagtatt tccccatgga agagtccacc tcaattaaga cagtttcttc 1501 cagaagaatt tatgaaaaca cttgagaaaa caggaccTCA GCTAACCTCT AGAATAAAAG 1561 GCGATTGGAT TGGACTTTAC CGGCATTTCC TAAAGTCTCC AAATTTTGAT GGCTGGTTTA 1621 AGACCCGGAG GAAGGAAATG ACCCAAAAAT TGGAGGCACT CCATCTAGAA GCTCTTTGTG 1681 AAGAGGACTT ACTTCTCTGG ATCCAGAAAC ACACAGAAGT AGAAACAGTA GACCTTGTCT 1741 TGAAGCTGAA AAATAAGCTG TTGCAGGCTG ATCGAGAGCA CTTACCTGTG AAACCTGACA 1801 CTATGGAAAA GTTACGGACA CACATAGATG CCATTATCTT AGCATTGCCA GAGGACTTGC 1861 AAGGCATACT GCTCAAAACG GGCATGACAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1921 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1981 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCGA 2041 AAGGTTTTTA CTTCCCAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2101 tgaaagatt