Construct: ORF TRCN0000470898

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006591.1_s317c1
Derived from:
ccsbBroadEn_05400
DNA Barcode:
CAAATGTTCTTCACCTATTCTTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF175 (285533)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470898
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285533 RNF175 ring finger protein 175 NM_173662.4 99.8% 99.6% 965T>A
2 human 285533 RNF175 ring finger protein 175 XM_005262938.3 89.5% 89.3% 764_765ins102;863T>A
3 human 285533 RNF175 ring finger protein 175 XM_011531879.2 87.8% 83.8% (many diffs)
4 human 285533 RNF175 ring finger protein 175 XM_011531881.2 87.7% 87.5% 0_1ins120;845T>A
5 human 285533 RNF175 ring finger protein 175 XM_011531882.2 79% 77.7% (many diffs)
6 human 285533 RNF175 ring finger protein 175 XM_011531883.2 75.7% 71.8% (many diffs)
7 human 285533 RNF175 ring finger protein 175 XM_005262939.3 75.4% 74.6% (many diffs)
8 human 285533 RNF175 ring finger protein 175 XM_005262940.4 75% 74.6% 0_1ins244;3delG;722T>A
9 human 285533 RNF175 ring finger protein 175 XM_017008047.1 69.7% 69.2% 104_105ins297;668T>A
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1050
ORF length:
984
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttagcatggc cgcggggacg gcggcgcgga aggcagcgcc ggtgctggag gcccccccgc
121agcaggagca gctctctcat acaaagcttt ctgcagaaga cacatggaac ctgcagcagg
181agaggatgta caagatgcac cggggccacg attccatgca cgtggaaatg atcttgatct
241tcctctgcgt tctggtcatt gcccagatag tgctggttca gtggagacag aggcatggcc
301gatcctacaa tctggtgacc ttgttgcaga tgtgggttgt ccccttatat ttcacgataa
361aattatactg gtggcggttt ctgtctatgt gggggatgtt ctccgttatt accagttaca
421tcctcttcag agctacccga aaacccctct caggaaggac accacgattg gtctacaaat
481ggtttctttt gatctacaaa ctcagctatg catttggtgt tgtgggttac ttggcgatca
541tgtttacaat gtgtggattc aatctgtttt tcaaaatcaa agctagagat tccatggatt
601ttggcattgt gtctttgttc tacggcctct actatggagt aatggggaga gactttgccg
661agatctgctc agactacatg gcttcCACTA TAGGGTTCTA CAGTGTCAGC CGGTTGCCTA
721CAAGGAGCTT ATCGGACAAT ATCTGTGCAG TCTGTGGGCA GAAGATCATT GTGGAGCTTG
781ATGAAGAAGG GCTCATTGAA AACACCTACC AGCTTTCCTG TAATCATGTC TTTCATGAAT
841TCTGCATCCG AGGTTGGTGT ATCGTTGGGA AAAAGCAGAC TTGCCCTTAC TGCAAAGAGA
901AAGTTGATTT GAAGAGGATG ATCAGTAATC CCTGGGAGCG CACACATTTT CTGTATGGAC
961AAATCCTGGA TTGGCTTCGT TATTTGGTGG CCTGGCAACC TGTGGTGATA GGAATAGTTC
1021AAGGCATTAA CTATTCACTA GGGCTGGAAT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1081TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1141CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAAAT
1201GTTCTTCACC TATTCTTGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1261tgaaagatt

Download FASTA (ORF) (Full)