Construct: ORF TRCN0000470902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013440.1_s317c1
- Derived from:
- ccsbBroadEn_04696
- DNA Barcode:
- TCTAATCACGCCCGAGCCTTTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUS1 (116150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116150 | NUS1 | NUS1 dehydrodolichyl diphos... | NM_138459.5 | 100% | 100% | |
| 2 | mouse | 52014 | Nus1 | NUS1 dehydrodolichyl diphos... | NM_030250.2 | 88.9% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 945
- ORF length:
- 879
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggggctgtac gagctggtgt ggcgggtgct gcacgcgctg ctctgtctgc 121 accgcacgct cacctcctgg ctccgcgttc ggttcggcac ctggaactgg atctggcggc 181 gctgctgccg cgccgcctct gccgcggtcc tagcgccgct cggcttcacg ctccgcaagc 241 ccccggcagt cggcaggaac cgccgtcacc accggcaccc gcgcgggggg tcgtgcctgg 301 cagccgcaca ccaccggatg cgctggcgcg cggacggtcg ttccttggag aagctgcctg 361 tgcatatggg cctggtgatc accgaggtgg agcaggaacc cagcttctcg gacatcgcga 421 gcctcgtggt gtggtgtatg gccgtgggca tctcctacat tagcgtctac gaccaccaag 481 gtattttcaa aagaaataat tccagattga tggatgaaat tttaaaacaa cagcaagaac 541 ttctgggccT AGATTGTTCA AAATACTCAC CAGAATTTGC AAATAGTAAT GACAAAGATG 601 ATCAAGTTTT AAATTGCCAT TTGGCAGTGA AGGTGCTGTC TCCGGAAGAT GGAAAAGCAG 661 ATATTGTAAG AGCTGCTCAG GACTTTTGCC AGTTAGTAGC CCAGAAGCAA AAGAGACCCA 721 CAGATTTGGA TGTAGATACG TTAGCCAGTT TACTTAGTTC AAATGGTTGT CCTGATCCTG 781 ATTTAGTATT GAAGTTCGGT CCTGTGGACA GCACATTAGG CTTTCTTCCC TGGCACATCA 841 GATTGACTGA GATTGTCTCT TTGCCTTCCC ACCTAAACAT CAGTTATGAG GACTTTTTCT 901 CTGCCCTTCG TCAATATGCA GCCTGTGAAC AGCGTCTGGG AAAGTACCCA ACTTTCTTGT 961 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1021 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1081 GAAAGGACGA TCTAATCACG CCCGAGCCTT TATCACGCGT TAAGTCgaca atcaacctct 1141 ggattacaaa atttgtgaaa gatt