Construct: ORF TRCN0000470905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006876.1_s317c1
- Derived from:
- ccsbBroadEn_02941
- DNA Barcode:
- ACGAGGCCTTTGGGCACCGACTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BLOC1S6 (26258)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NM_012388.3 | 100% | 100% | |
| 2 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NM_001311255.1 | 86.2% | 80.9% | (many diffs) |
| 3 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NM_001311256.1 | 39.7% | 29.3% | (many diffs) |
| 4 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132356.1 | 12.7% | (many diffs) | |
| 5 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132351.1 | 12.6% | 1_321del;545_546insACAG;834_4056del | |
| 6 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132350.1 | 12.3% | (many diffs) | |
| 7 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132352.1 | 10.5% | 1_321del;545_546ins88;750_3972del | |
| 8 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132357.1 | 10.4% | (many diffs) | |
| 9 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132353.1 | 9.2% | 1_321del;403_404ins142;696_3918del | |
| 10 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132354.1 | 9.1% | 1_321del;402_403ins146;692_3914del | |
| 11 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132359.1 | 7.4% | (many diffs) | |
| 12 | human | 26258 | BLOC1S6 | biogenesis of lysosomal org... | NR_132355.1 | 7% | 1_321del;401_402ins230;608_3830del | |
| 13 | mouse | 18457 | Bloc1s6 | biogenesis of lysosomal org... | NM_019788.3 | 82.1% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgtccctggg ccgtcgtctc cggacggggc cctgacacgg ccaccctact 121 gcctggaggc cggggagccg acgcctggtt taagtgacac ttctccagat gaagggttaa 181 tagaggactt GACTATAGAA GACAAAGCAG TGGAGCAACT GGCAGAAGGA TTGCTTTCTC 241 ATTATTTGCC AGATCTGCAG AGATCAAAAC AAGCCCTCCA GGAACTCACA CAGAACCAAG 301 TTGTATTGTT AGACACACTG GAACAAGAGA TTTCAAAATT TAAAGAATGT CATTCTATGT 361 TGGATATTAA TGCTTTGTTT GCTGAGGCTA AACACTATCA TGCCAAGTTG GTGAATATAA 421 GAAAAGAGAT GCTGATGCTT CATGAAAAAA CATCAAAGTT AAAAAAAAGA GCACTTAAAC 481 TGCAGCAGAA GAGGCAAAAA GAAGAGTTGG AAAGGGAGCA GCAACGAGAG AAGGAGTTTG 541 AAAGAGAAAA GCAGTTAACT GCCAGACCAG CCAAAAGGAT GTGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAACG AGGCCTTTGG GCACCGACTA AACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t