Construct: ORF TRCN0000470909
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018040.1_s317c1
- Derived from:
- ccsbBroadEn_04620
- DNA Barcode:
- TCCATAAACAGCCGTATAATACCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AZ2 (94239)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470909
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_012412.4 | 100% | 100% | |
| 2 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_138635.3 | 83.7% | 85.1% | 325_334delGAGAAGAGAA;340_341delTCinsGA;342_343ins52 |
| 3 | human | 3015 | H2AZ1 | H2A.Z variant histone 1 | NM_002106.4 | 80.8% | 97.6% | (many diffs) |
| 4 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_201436.2 | 79.2% | 78.9% | 0_1ins79;2delT |
| 5 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_201517.2 | 70.3% | 70.3% | 79_80ins114 |
| 6 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_201516.2 | 51.5% | 51.5% | 198_199ins186 |
| 7 | mouse | 77605 | H2afv | H2A histone family, member V | NM_029938.1 | 91.6% | 100% | (many diffs) |
| 8 | mouse | 77605 | H2afv | H2A histone family, member V | XM_017314836.1 | 81.5% | 75.9% | (many diffs) |
| 9 | mouse | 51788 | H2afz | H2A histone family, member Z | NM_016750.3 | 81.2% | 97.6% | (many diffs) |
| 10 | mouse | 51788 | H2afz | H2A histone family, member Z | NM_001316995.1 | 66.4% | 79.6% | (many diffs) |
| 11 | mouse | 77605 | H2afv | H2A histone family, member V | NM_001347064.1 | 63.8% | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggaggcaaa gctggaaagg acagtgggaa ggccaaggct aaggcagtat 121 ctcgctcaca gagagctggg ctacagtttc ctgtgggccg catccacaga cacttGAAGA 181 CTCGCACCAC AAGCCATGGA AGGGTGGGTG CCACTGCTGC CGTGTACAGT GCTGCGATTC 241 TGGAGTACCT CACTGCAGAG GTGCTGGAGC TGGCAGGTAA TGCTTCTAAG GATCTCAAAG 301 TAAAGCGTAT CACTCCGCGT CACTTGCAGC TTGCAATCCG TGGTGATGAA GAGTTGGATT 361 CTCTTATCAA GGCTACCATA GCTGGGGGTG GTGTGATCCC TCACATCCAC AAATCTCTGA 421 TTGGAAAGAA GGGACAGCAG AAAACTGCTT ACCAACTTTC TTGTACAAAG TGGTTGATAT 481 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 541 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCATA 601 AACAGCCGTA TAATACCAAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 661 gaaagatt