Construct: ORF TRCN0000470909

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018040.1_s317c1
Derived from:
ccsbBroadEn_04620
DNA Barcode:
TCCATAAACAGCCGTATAATACCA
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
H2AZ2 (94239)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470909
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 94239 H2AZ2 H2A.Z variant histone 2 NM_012412.4 100% 100%
2 human 94239 H2AZ2 H2A.Z variant histone 2 NM_138635.3 83.7% 85.1% 325_334delGAGAAGAGAA;340_341delTCinsGA;342_343ins52
3 human 3015 H2AZ1 H2A.Z variant histone 1 NM_002106.4 80.8% 97.6% (many diffs)
4 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201436.2 79.2% 78.9% 0_1ins79;2delT
5 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201517.2 70.3% 70.3% 79_80ins114
6 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201516.2 51.5% 51.5% 198_199ins186
7 mouse 77605 H2afv H2A histone family, member V NM_029938.1 91.6% 100% (many diffs)
8 mouse 77605 H2afv H2A histone family, member V XM_017314836.1 81.5% 75.9% (many diffs)
9 mouse 51788 H2afz H2A histone family, member Z NM_016750.3 81.2% 97.6% (many diffs)
10 mouse 51788 H2afz H2A histone family, member Z NM_001316995.1 66.4% 79.6% (many diffs)
11 mouse 77605 H2afv H2A histone family, member V NM_001347064.1 63.8% 70.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
450
ORF length:
384
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tggaggcaaa gctggaaagg acagtgggaa ggccaaggct aaggcagtat
121ctcgctcaca gagagctggg ctacagtttc ctgtgggccg catccacaga cacttGAAGA
181CTCGCACCAC AAGCCATGGA AGGGTGGGTG CCACTGCTGC CGTGTACAGT GCTGCGATTC
241TGGAGTACCT CACTGCAGAG GTGCTGGAGC TGGCAGGTAA TGCTTCTAAG GATCTCAAAG
301TAAAGCGTAT CACTCCGCGT CACTTGCAGC TTGCAATCCG TGGTGATGAA GAGTTGGATT
361CTCTTATCAA GGCTACCATA GCTGGGGGTG GTGTGATCCC TCACATCCAC AAATCTCTGA
421TTGGAAAGAA GGGACAGCAG AAAACTGCTT ACCAACTTTC TTGTACAAAG TGGTTGATAT
481CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC
541GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCATA
601AACAGCCGTA TAATACCAAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
661gaaagatt

Download FASTA (ORF) (Full)