Construct: ORF TRCN0000470914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018017.1_s317c1
- Derived from:
- ccsbBroadEn_02881
- DNA Barcode:
- CGCTCAGCAAGTTGCCGCTCGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLFML2B (25903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25903 | OLFML2B | olfactomedin like 2B | NM_015441.2 | 99.9% | 100% | 576T>C |
| 2 | human | 25903 | OLFML2B | olfactomedin like 2B | NM_001297713.1 | 99.8% | 99.8% | 576T>C;722_724delAGC |
| 3 | human | 25903 | OLFML2B | olfactomedin like 2B | NM_001347700.1 | 99.6% | 99.7% | 576T>C;722_727delAGGAGC |
| 4 | human | 25903 | OLFML2B | olfactomedin like 2B | XM_017000967.2 | 68.6% | 63.2% | (many diffs) |
| 5 | human | 25903 | OLFML2B | olfactomedin like 2B | XM_011509398.2 | 67.9% | 67.8% | 0_1ins721;2delT |
| 6 | mouse | 320078 | Olfml2b | olfactomedin-like 2B | NM_177068.4 | 82.9% | 82.6% | (many diffs) |
| 7 | mouse | 320078 | Olfml2b | olfactomedin-like 2B | XM_006496901.2 | 82.7% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2316
- ORF length:
- 2250
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagcctcgg ctgctagttc tctacttcgc tctgattgtg gttccggcct 121 gggtgtccag cattgtcctc acagggacaa gcgagccccc agatgcgcag acagtggcgc 181 ctgcggagga cgagactctg caaaacgagg cggacaacca ggagaacgtt ttatctcagt 241 tgctggggga ctatgacaag gtcaaggcta tgtctgaggg ctcggactgt cagtgcaagt 301 gtgtggtgag acccctgggc cgggatgcct gccagaggat caatgcgggg gcctccagga 361 aggaagactt ctataccgtg gaaaccatca cctcaggctc gtcgtgcaag tgtgcctgtg 421 tagcaccccc atcggccctc aatccctgcg agggagactt caggctccag aagctgcggg 481 aggcagacag ccaggacttg aagctctcca caatcataga catgttggaa ggagcgttct 541 atggcctgga tctcctgaag ctacattcag tcaccaccaa actggtgggg cgagtggata 601 aactggagga ggaagtgtct aaaaacctca ccaaggaaaa cgaacaaatc aaagaggaca 661 tggaagaaat tcgaaccgag atgaataagc gaggcaaaga aaattgctct gaaaacatcc 721 tagatagcat gccagacatc cgctcagccc tgcagaggga tgcagcagca gcctacgccc 781 acccagagta tgaagagcgg tttctgcagg aagaaaccgt gtcccagcag atcaactcca 841 tcgaacttct gcagacgcga cccctggctc tgcctgaggt ggtgaagtca cagcggcccc 901 tgcagaggca ggtccacctg agaggccggc cggcctccca gcccactgtc atccggggca 961 tcacctacta taaagccaag gtctctgaag aagagaatga cattgaagag cagcaagatg 1021 agtttttcag cggtgacaat ggagtggatt tgctgattga agatcagctc ctgagacaca 1081 acggcctgat gaccagtgtc acccggaggc ctgcagccac ccgtcaggga cacagcactg 1141 ctgtgacaag cgacctgaac gctcggaccg caccctggtc ctcagcactg ccacagccct 1201 cgacctcaga tcccagcatc gccaaccatg cctcagtggg accaacactc caaacaacct 1261 cggtgtctcc agatcccaca agggagtcag tcctgcagcc ttctcctcag gtaccagcca 1321 ccactgtggc ccacacagcc acccagcaac cagcagcccc agctcctccg gcagtgtctc 1381 ccagggaggc attgatggaa gctatgcaca cagtcccagt gcctcccacc acagtcagaa 1441 cagactcgct ggggaaagat gctcctgctg ggtggggaac aacccctgcc agccccacgc 1501 tgagccccga agaagaagat gacatccgga atgtcatagg aaggtgcaag gacactctct 1561 ccacaatcac ggggccgacc acccagaaca catatgggcg gaatgaaggg gcctggatga 1621 aggaccccct ggccaaggat gagcggattt acgtaaccaa ctattactac ggcaacaccc 1681 tggtagagtt ccggaacctg gagaacttca aacaaggtcg ctggagcaat tcctacaagc 1741 tcccgtacag ctggatcggc acaggccacg tggtatacaa tggcgccttc tactacaatc 1801 gcgccttcac ccgcaacatc atcaagtacg acctgaagca gcgctacgtg gctgcctggg 1861 ccatgctgca tgacgtggcc tacgaggagg ccaccccctg gcgatggcag ggccactcag 1921 acgtggactt tgctgtggac gagaatggcc TATGGCTCAT CTACCCGGCC CTGGACGATG 1981 AGGGCTTCAG CCAGGAGGTC ATTGTCCTGA GCAAGCTCAA TGCCGCGGAC CTGAGCACAC 2041 AGAAGGAGAC CACATGGCGC ACGGGGCTCC GGAGGAATTT CTACGGCAAC TGCTTCGTCA 2101 TCTGTGGGGT GCTGTATGCC GTGGATAGCT ACAACCAGCG GAATGCCAAC ATCTCCTACG 2161 CTTTCGACAC CCACACCAAC ACACAGATCG TCCCCAGGCT GCTGTTCGAG AATGAGTATT 2221 CCTATACGAC CCAGATAGAC TACAACCCCA AGGACCGCCT GCTCTATGCC TGGGACAATG 2281 GCCACCAGGT CACTTACCAT GTCATCTTTG CCTACTGCCC AACTTTCTTG TACAAAGTGG 2341 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2401 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2461 ACGCTCAGCA AGTTGCCGCT CGAGAACGCG TTAAGTCgac aatcaacctc tggattacaa 2521 aatttgtgaa agatt