Construct: ORF TRCN0000470922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013149.1_s317c1
- Derived from:
- ccsbBroadEn_00718
- DNA Barcode:
- GCGTCATGAGTAACACGTACTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AZ1 (3015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470922
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3015 | H2AZ1 | H2A.Z variant histone 1 | NM_002106.4 | 100% | 100% | |
| 2 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_012412.4 | 80.8% | 97.6% | (many diffs) |
| 3 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_201436.2 | 64.5% | 77.3% | (many diffs) |
| 4 | human | 94239 | H2AZ2 | H2A.Z variant histone 2 | NM_201517.2 | 59% | 68.7% | (many diffs) |
| 5 | mouse | 51788 | H2afz | H2A histone family, member Z | NM_016750.3 | 94.7% | 100% | (many diffs) |
| 6 | mouse | 51788 | H2afz | H2A histone family, member Z | NM_001316995.1 | 78.1% | 82% | (many diffs) |
| 7 | mouse | 666634 | Gm8203 | predicted pseudogene 8203 | XR_105012.5 | 41% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggcggtaag gctggaaagg actccggaaa ggccaagaca aaggcggTTT 121 CCCGCTCGCA GAGAGCCGGC TTGCAGTTCC CAGTGGGCCG TATTCATCGA CACCTAAAAT 181 CTAGGACGAC CAGTCATGGA CGTGTGGGCG CGACTGCCGC TGTGTACAGC GCAGCCATCC 241 TGGAGTACCT CACCGCAGAG GTACTTGAAC TGGCAGGAAA TGCATCAAAA GACTTAAAGG 301 TAAAGCGTAT TACCCCTCGT CACTTGCAAC TTGCTATTCG TGGAGATGAA GAATTGGATT 361 CTCTCATCAA GGCTACAATT GCTGGTGGTG GTGTCATTCC ACACATCCAC AAATCTCTGA 421 TTGGGAAGAA AGGACAACAG AAGACTGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGTC 601 ATGAGTAACA CGTACTCTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt