Construct: ORF TRCN0000470922

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013149.1_s317c1
Derived from:
ccsbBroadEn_00718
DNA Barcode:
GCGTCATGAGTAACACGTACTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
H2AZ1 (3015)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470922
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3015 H2AZ1 H2A.Z variant histone 1 NM_002106.4 100% 100%
2 human 94239 H2AZ2 H2A.Z variant histone 2 NM_012412.4 80.8% 97.6% (many diffs)
3 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201436.2 64.5% 77.3% (many diffs)
4 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201517.2 59% 68.7% (many diffs)
5 mouse 51788 H2afz H2A histone family, member Z NM_016750.3 94.7% 100% (many diffs)
6 mouse 51788 H2afz H2A histone family, member Z NM_001316995.1 78.1% 82% (many diffs)
7 mouse 666634 Gm8203 predicted pseudogene 8203 XR_105012.5 41% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
450
ORF length:
384
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tggcggtaag gctggaaagg actccggaaa ggccaagaca aaggcggTTT
121CCCGCTCGCA GAGAGCCGGC TTGCAGTTCC CAGTGGGCCG TATTCATCGA CACCTAAAAT
181CTAGGACGAC CAGTCATGGA CGTGTGGGCG CGACTGCCGC TGTGTACAGC GCAGCCATCC
241TGGAGTACCT CACCGCAGAG GTACTTGAAC TGGCAGGAAA TGCATCAAAA GACTTAAAGG
301TAAAGCGTAT TACCCCTCGT CACTTGCAAC TTGCTATTCG TGGAGATGAA GAATTGGATT
361CTCTCATCAA GGCTACAATT GCTGGTGGTG GTGTCATTCC ACACATCCAC AAATCTCTGA
421TTGGGAAGAA AGGACAACAG AAGACTGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA
481TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
541CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGTC
601ATGAGTAACA CGTACTCTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
661tgaaagatt

Download FASTA (ORF) (Full)