Construct: ORF TRCN0000470932
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005986.1_s317c1
- Derived from:
- ccsbBroadEn_04206
- DNA Barcode:
- AAGGGCGCTTGCCAACTGACCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GKAP1 (80318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470932
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | NM_025211.4 | 100% | 100% | |
| 2 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_005252241.2 | 100% | 100% | |
| 3 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_011519058.2 | 100% | 100% | |
| 4 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | NM_001135953.1 | 86% | 86% | 583_584ins153 |
| 5 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_006717293.1 | 66.1% | 66.1% | 0_1ins372 |
| 6 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_017015171.1 | 66.1% | 66.1% | 0_1ins372 |
| 7 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | NM_019832.4 | 85.3% | 87.9% | (many diffs) |
| 8 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_006517301.3 | 85.3% | 87.9% | (many diffs) |
| 9 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_011244542.2 | 85.3% | 87.9% | (many diffs) |
| 10 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_011244543.2 | 85.3% | 87.9% | (many diffs) |
| 11 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_006517304.3 | 77.1% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1164
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcagcagta cttagttctg ttcccaccac cgcttctcgt tttgccctgt 121 tacaagtgga tagtggcagt ggctctgatt ctgaacctgg aaaaggtaaa ggtcgaaata 181 ctggaaagtc tcaaacttta ggaagcaagt caactacaaa tgagaaaaaa agagagaaaa 241 gaagaaaaaa gaaggaacag caacagagtg aagcaaatga gctcaggaat cttgctttta 301 agaaaattcc ccagaaatcc tcccatgctg tttgtaacgc tcaacatgat cttccattgt 361 caaacccagt acagaaggat tcacgagaag aaaattggca agagtggaga caaagagatg 421 agcagctgac atctgaaatg tttgaagcag atcttgagaa ggcattgtta ctaagtaaac 481 tagaatatga agagcacaaa aaggagtatg aagatgctga aaatacttca actcagtcca 541 aagttatgaa taaaaaagat aaaagaaaga atcatcaggg aaaagacaga cctctcacag 601 tatcactaaa agattttcat tcggaagatc acattagtaa aaagactgag gaattgagtt 661 cttctcagac tttatcacat gatggAGGAT TCTTCAATAG ACTGGAAGAT GATGTTCATA 721 AAATTCTTAT TAGAGAAAAA CGAAGAGAAC AGCTTACAGA ATATAATGGA ACAGATAATT 781 GTACAGCTCA TGAACACAAC CAGGAAGTGG TTCTGAAAGA TGGAAGAATT GAAAGACTAA 841 AGTTAGAGCT TGAAAGGAAA GATGCTGAAA TCCAGAAGCT GAAAAATGTA ATCACTCAAT 901 GGGAGGCAAA GTATAAGGAA GTAAAGGCAA GAAATGCACA ATTATTGAAA ATGCTTCAGG 961 AAGGTGAAAT GAAAGATAAG GCAGAAATAC TTCTGCAAGT TGATGAATCA CAAAGTATCA 1021 AGAATGAGCT CACTATTCAG GTGACTTCAC TTCATGCTGC ATTAGAACAA GAAAGATCTA 1081 AAGTGAAAGT ATTACAAGCA GAGTTAGCCA AATACCAGGG TGGCAGAAAA GGGAAAAGAA 1141 ACTCTGAATC CGACCAGTGT AGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGGGCGCTTG 1321 CCAACTGACC GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att