Construct: ORF TRCN0000470932

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005986.1_s317c1
Derived from:
ccsbBroadEn_04206
DNA Barcode:
AAGGGCGCTTGCCAACTGACCGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GKAP1 (80318)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470932
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80318 GKAP1 G kinase anchoring protein 1 NM_025211.4 100% 100%
2 human 80318 GKAP1 G kinase anchoring protein 1 XM_005252241.2 100% 100%
3 human 80318 GKAP1 G kinase anchoring protein 1 XM_011519058.2 100% 100%
4 human 80318 GKAP1 G kinase anchoring protein 1 NM_001135953.1 86% 86% 583_584ins153
5 human 80318 GKAP1 G kinase anchoring protein 1 XM_006717293.1 66.1% 66.1% 0_1ins372
6 human 80318 GKAP1 G kinase anchoring protein 1 XM_017015171.1 66.1% 66.1% 0_1ins372
7 mouse 56278 Gkap1 G kinase anchoring protein 1 NM_019832.4 85.3% 87.9% (many diffs)
8 mouse 56278 Gkap1 G kinase anchoring protein 1 XM_006517301.3 85.3% 87.9% (many diffs)
9 mouse 56278 Gkap1 G kinase anchoring protein 1 XM_011244542.2 85.3% 87.9% (many diffs)
10 mouse 56278 Gkap1 G kinase anchoring protein 1 XM_011244543.2 85.3% 87.9% (many diffs)
11 mouse 56278 Gkap1 G kinase anchoring protein 1 XM_006517304.3 77.1% 79.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1164
ORF length:
1098
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ctcagcagta cttagttctg ttcccaccac cgcttctcgt tttgccctgt
121tacaagtgga tagtggcagt ggctctgatt ctgaacctgg aaaaggtaaa ggtcgaaata
181ctggaaagtc tcaaacttta ggaagcaagt caactacaaa tgagaaaaaa agagagaaaa
241gaagaaaaaa gaaggaacag caacagagtg aagcaaatga gctcaggaat cttgctttta
301agaaaattcc ccagaaatcc tcccatgctg tttgtaacgc tcaacatgat cttccattgt
361caaacccagt acagaaggat tcacgagaag aaaattggca agagtggaga caaagagatg
421agcagctgac atctgaaatg tttgaagcag atcttgagaa ggcattgtta ctaagtaaac
481tagaatatga agagcacaaa aaggagtatg aagatgctga aaatacttca actcagtcca
541aagttatgaa taaaaaagat aaaagaaaga atcatcaggg aaaagacaga cctctcacag
601tatcactaaa agattttcat tcggaagatc acattagtaa aaagactgag gaattgagtt
661cttctcagac tttatcacat gatggAGGAT TCTTCAATAG ACTGGAAGAT GATGTTCATA
721AAATTCTTAT TAGAGAAAAA CGAAGAGAAC AGCTTACAGA ATATAATGGA ACAGATAATT
781GTACAGCTCA TGAACACAAC CAGGAAGTGG TTCTGAAAGA TGGAAGAATT GAAAGACTAA
841AGTTAGAGCT TGAAAGGAAA GATGCTGAAA TCCAGAAGCT GAAAAATGTA ATCACTCAAT
901GGGAGGCAAA GTATAAGGAA GTAAAGGCAA GAAATGCACA ATTATTGAAA ATGCTTCAGG
961AAGGTGAAAT GAAAGATAAG GCAGAAATAC TTCTGCAAGT TGATGAATCA CAAAGTATCA
1021AGAATGAGCT CACTATTCAG GTGACTTCAC TTCATGCTGC ATTAGAACAA GAAAGATCTA
1081AAGTGAAAGT ATTACAAGCA GAGTTAGCCA AATACCAGGG TGGCAGAAAA GGGAAAAGAA
1141ACTCTGAATC CGACCAGTGT AGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1201AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1261TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGGGCGCTTG
1321CCAACTGACC GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1381att

Download FASTA (ORF) (Full)