Construct: ORF TRCN0000470954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004843.1_s317c1
- Derived from:
- ccsbBroadEn_13729
- DNA Barcode:
- GGCGTCGGCGCACGAACGTAGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL23AP64 (649946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 649946 | RPL23AP64 | ribosomal protein L23a pseu... | NR_003040.2 | 36.3% | (many diffs) | |
| 2 | human | 6147 | RPL23A | ribosomal protein L23a | NM_000984.6 | 35% | 32.3% | (many diffs) |
| 3 | human | 56969 | RPL23AP32 | ribosomal protein L23a pseu... | NR_002229.2 | 31.1% | (many diffs) | |
| 4 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026973.1 | 30.7% | (many diffs) | |
| 5 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_000029.3 | 17.5% | (many diffs) | |
| 6 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024529.1 | 15.5% | (many diffs) | |
| 7 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026982.1 | 14.9% | (many diffs) | |
| 8 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024528.1 | 14.5% | (many diffs) | |
| 9 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026981.1 | 14.2% | (many diffs) | |
| 10 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026972.1 | 11.2% | (many diffs) | |
| 11 | human | 388574 | RPL23AP87 | ribosomal protein L23a pseu... | NR_029406.1 | 9.5% | (many diffs) | |
| 12 | human | 644128 | RPL23AP53 | ribosomal protein L23a pseu... | NR_003572.2 | 3.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 243
- ORF end:
- 486
- ORF length:
- 243
- Sequence:
-
1 TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG 61 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 121 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 181 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 241 GCATGAAGAA GACAGAAGAC AACAACGCAC TTGTGTTCAC TGTGAATGTT AAAGCCAACA 301 AGCACCAGAA GAAGCTCTAT GACACTGATG TGGCCAAGGT CAACACCCTG ATTCGGCCTG 361 ATGGAGAGAA GGAACATGTT CGACTGGCTC CTGATTACAA TGCTTTGGAT GTTGCCAACA 421 AAATAGGGAT CATCTCAACT GAGTCCAGTT GGCTAATTCT AAATATATGT ATATCTTTTC 481 ACCATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGCGTCGGC GCACGAACGT AGATGACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt