Construct: ORF TRCN0000470965

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002918.1_s317c1
Derived from:
ccsbBroadEn_14314
DNA Barcode:
ATATCTTGGGGATCAGTCCGGGCA
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLEKHA8 (84725)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470965
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84725 PLEKHA8 pleckstrin homology domain ... NM_032639.4 99.9% 98.8% 1306delC
2 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001363473.1 98.7% 98.4% (many diffs)
3 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001350974.2 97.6% 98.4% 1301_1302insAAATCAACAGAAAACAC;1303_1317del
4 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001350973.2 96.1% 96% (many diffs)
5 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001197027.1 95.2% 95.2% (many diffs)
6 human 84725 PLEKHA8 pleckstrin homology domain ... XM_005249897.2 94.9% 94.5% (many diffs)
7 human 84725 PLEKHA8 pleckstrin homology domain ... XM_011515592.3 92.5% 92.4% 1301_1362del;1368delC;1383_1425del
8 human 84725 PLEKHA8 pleckstrin homology domain ... XM_011515591.2 89% 89% (many diffs)
9 human 84725 PLEKHA8 pleckstrin homology domain ... XM_017012744.1 89% 89% (many diffs)
10 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001197026.2 84.2% 84.2% (many diffs)
11 human 51054 PLEKHA8P1 pleckstrin homology domain ... NR_037144.1 65.6% (many diffs)
12 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001363474.1 58.1% 57.7% (many diffs)
13 human 84725 PLEKHA8 pleckstrin homology domain ... XM_011515596.2 51.7% 51.6% (many diffs)
14 human 84725 PLEKHA8 pleckstrin homology domain ... NM_001350975.1 51.6% 51.1% (many diffs)
15 human 84725 PLEKHA8 pleckstrin homology domain ... XM_017012746.1 45.9% 45.8% (many diffs)
16 human 84725 PLEKHA8 pleckstrin homology domain ... XM_017012747.1 45.9% 45.8% (many diffs)
17 mouse 231999 Plekha8 pleckstrin homology domain ... NM_001164361.1 73.7% 77.8% (many diffs)
18 mouse 231999 Plekha8 pleckstrin homology domain ... NM_001001335.2 65.6% 69.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1386
ORF length:
1320
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gggggtgctg tacaagtgga ccaactatct gagcggttgg cagcctcgat
121ggttccttct ctgtggggga atattgtcct attatgattc tcctgaagat gcctggaaag
181gttgcaaagg gagcatacaa atggcagtct gtgaaattca agttcattct gtagataata
241cacgcatgga cctgataatc cctggggaac agtatttcta cctgaaggcc agaagtgtgg
301ctgaaagaca gcggtggctg gtggccctgg gatcagccaa ggcttgcctg actgacagta
361ggacccagaa ggagaaagag tttgctgaaa acactgaaaa cttgaaaacc aaaatgtcag
421aactaagact ctactgtgac ctccttgttc agcaagtaga taaaacaaaa gaagtgacca
481caactggtgt gtccaattct gaggagggaa ttgatgtggg aactttgctg aaatcaacct
541gtaatacttt tctgaagacc ttggaagaat gcatgcagat cgcaaatgca gccttcacct
601ctgagctgct ctaccgcact ccaccaggat cacctcagct ggccatgctc aagtccagca
661agatgaaaca tcctattata ccaattcata attcattgga aaggcaaatg gagttgagca
721cttgtgaaaa tggatcttta aatatggaaa taaatggtga ggaagaaatc ctaatgaaaa
781ataagaattc cttatatttg aaatctgcag agatagactg cagcatatca agtgaggaaa
841atacagatga taatataaca gtccaaggtg aaataaggaa ggaagatgga atggaaaacc
901tgaaaaatca tgacaataac ttgactcagt ctggatcaga ctcaagttgc tcTCCGGAAT
961GCCTCTGGGA GGAAGGCAAA GAAGTTATCC CAACTTTCTT TAGTACCATG AACACAAGCT
1021TTAGTGACAT TGAACTTCTG GAAGACAGTG GCATTCCCAC AGAAGCATTC TTGGCATCAT
1081GTTATGCTGT GGTTCCAGTA TTAGACAAAC TTGGCCCTAC AGTGTTTGCT CCTGTTAAGA
1141TGGATCTTGT TGGAAATATT AAGAAAGTAA ATCAGAAGTA TATAACCAAC AAAGAAGAGT
1201TTACCACTCT CCAGAAGATA GTGCTGCACG AAGTGGAGGC GGATGTAGCC CAGGTTAGGA
1261ACTCAGCGAC TGAAGCCCTC TTGTGGCTGA AGAGAGGTCT CAAATTTTTG AAGGGATTTT
1321TGACAGAAGT GAAAAATGGG GAGAAGGATA TCCAGACAGC CCTAAGAAAT CAACAGAAAA
1381CACTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT
1441CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC
1501TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATATCTTGGG GATCAGTCCG GGCAACGCGT
1561TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)